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Genetic Engineering and Biotechnology. Created by: Evelin Fuentes Rai Boone . Genetic Engineering 4.4.7. Genetic engineering : the deliberate manipulation of genetic material.

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genetic engineering and biotechnology

Genetic Engineering and Biotechnology

Created by: Evelin Fuentes

Rai Boone

genetic engineering 4 4 7
Genetic Engineering 4.4.7
  • Genetic engineering: the deliberate manipulation of genetic material.
  • Genetic code: The sequence of nucleotides in DNA or RNA that determines the specific amino acid sequence in the synthesis of proteins.
genetic engineering
Genetic Engineering
  • It is possible to move material between species because the genetic code is universal.
  • The transfer of genetic code can cause genetic mutations in animals
gene transfer 4 4 8
Gene Transfer 4.4.8
  • Gene transfer: Involves the use of plasmids, host cell (bacterium, yeast, or other cell), restriction enzymes (endonucleases), DNA ligase.
  • Gene therapy: The treatment of certain diseases by introducing specific engineered genes in a patients cells.
  • Vector: Is what is needed to carry the gene into the host cell; plasmids are often used as vectors.
  • Restriction enzyme: Used to cut a desired section of the DNA.
  • Plasmids: Used to clone a desired gene.
  • Host cell: The cell used to receive the genetic material.
the use of gene transfer for diabetics
The use of gene transfer for diabetics
  • A human gene that codes for insulin can be inserted into a plasmid and then inserted into a host cell (bacterium). The bacterium then synthesis insulin which can then be collected and used by diabetics.
slide6

Note- Gene transfer involves the following elements: a vector, a host cell, restriction enzyme, DNA ligase.

  • Step 1: Introduce the desired gene into a plasmid and transfer it into a bacterial cell, then culture the bacteria.
    • Note: many of the bacteria will have a plasmid with the desired gene
  • Step 2: Use restriction enzymes to cut the desired gene out of the plasmids and purify the gene using gel electrophoresis.
works cited
Works Cited
  • "Genetic Code: Definition from Answers.com." Answers.com: Wiki Q&A Combined with Free Online Dictionary, Thesaurus, and Encyclopedias. Web. 13 Feb. 2012. <http://www.answers.com/topic/genetic-code>.
  • "IB Biology Notes - Genetic Engineering And Biotechnology." IB Guides - Free International Baccalaureate Study Guides, Notes, Videos and Powerpoints. Web. 13 Feb. 2012. <http://www.ibguides.com/biology/notes/genetic-engineering-and-biotechnology>.
  • "Gene Therapy: Definition from Answers.com." Answers.com: Wiki Q&A Combined with Free Online Dictionary, Thesaurus, and Encyclopedias. Web. 13 Feb. 2012. <http://www.answers.com/topic/gene-therapy>.
  • "BrainPOP | Science." BrainPOP - Animated Educational Site for Kids - Science, Social Studies, English, Math, Arts. Web. 13 Feb. 2012. <http://www.brainpop.com/science/>.
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