Ultraviolet A exposure might increase metastasis of mouse melanoma: a pilot study Pastilla et al. Photodermatol. Photoimmunol Photomed 2005; 21: 183-190. Introduction. UVA radiation (320-400nm) somes from excessive sunbathing and tanning in solaria.
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Ultraviolet A exposure might increase metastasis of mouse melanoma: a pilot studyPastilla et al.Photodermatol. Photoimmunol Photomed 2005; 21: 183-190
-Original Philips UVA facial tanner lamp and 5mm thick UVB glass filter.
-Irradiances were determined by a double holographic grating spectroradiometer. It was calibrated against a 1000W halogen standard lamp.
-The UVA spectrum that reached the lamp was 310-400nm; reaching UVA spectrum was 99.9% and UVB was 0.1%.
Animal experiment:-C56BL/6 mice 8-10 weeks of age. Groups of 10 mice.
-Hair on the abdomen shaved for UVA irradiation.
-Suspension of 50000 B16-F1 or B16-F10 cells injected in the tail vein.
-Immediate 8J/cm2 of UVA. Some animals were exposed to two more doses (UVA 8J/cm2) on two consecutive post-injection days.
-A control group received the B16-F10 cells but not the UVA, a group only received saline.
-Mice terminated 14 days post-injection, lungs extracted and fixed (Bouuin), then dissection with a dissecting microscope.
-After qualitative evaluation, paraffin, sections in 5microns, hematoxyline-eosin, Melan A, N-cadherin, E-cadherin.