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W 4/23 EXAM III Chapters 4,13-16,18

W 4/23 EXAM III Chapters 4,13-16,18 M 4/28 Biotechnology W 4/30 Biotechnology and review for Final Exam Biotechnology topics: 1) DNA sequencing Figure 20.1 plus the related text

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W 4/23 EXAM III Chapters 4,13-16,18

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  1. W 4/23 EXAM III Chapters 4,13-16,18 • M 4/28 Biotechnology • W 4/30 Biotechnology and review for Final Exam • Biotechnology topics: 1) DNA sequencing Figure 20.1 plus the related text 2) Green Fluorescent Protein = GFP not in textbook • 3) PCR Figure 19.5 plus the related text • Cumulative Final Exam: • Wednesday, May 7th, 10:45-12:45, • room 303 Payson-Smith • Do not bring any of your • lecture notes to hand in at the • Final Exam.

  2. 1) All living things are composed of cells* • 2) All cells come from pre-existing cells* • * The Cell Theory • 3) All cells exist in a water environment 4) All cells are bounded by a membrane • 5) All cells have similar chemistrys (proteins, proteins, proteins) 6) DNA is the primary heritable molecule (DNA’s BIG job is to code for proteins) • 7) The concept of gene regulation and totipotency

  3. 1) DNA sequencing Figure 20.1 plus the related text

  4. This is the procedure for DNA sequencing.

  5. BLAST! search at NCBIhttp://www.ncbi.nlm.nih.gov/National Center for Biotechnology Information

  6. BLASTing the genome to search for a match with the human insulin gene gives the following result:

  7. We can BLAST the fruit fly genome with human insulin and we find an ortholog:

  8. BLASTing the genome to search for a match with the human insulin gene gives the following result:

  9. When we BLAST the human genome with the insulin gene, we Find the insulin gene and we also find two other genes that are very similar to insulin:

  10. The major hormones that regulate growth in animals are insulin and insulin-like growth factor (the insulin signaling system.)

  11. 100+ *** 100 * 98 96 * A 94 *** 92 _______ 90 * 88 * A- 86 * _______ 84 **** B+ 82 *** 80 ** _______ 78 ****** B 76 ***** MEAN = 77 74 ** 72 _______ 70 * 68 *** C 66 _______ 64 ** 62 **** C- 60 ** 58 *** _______ 56 54 * 52 50 48 46 ** D- 44 42

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