Pls 623 physiology of plants ii spring semester 2014
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PLS 623: Physiology of Plants II Spring Semester 2014. Jan Smalle ( [email protected] ). Journal club format. Why? Practice how to prepare papers and express ideas correctly. Training in science debate . Practice presentation: research seminar. TARGETED PROTEIN DEGRADATION.

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PLS 623: Physiology of Plants II Spring Semester 2014

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Pls 623 physiology of plants ii spring semester 2014

PLS 623: Physiology of Plants IISpring Semester 2014

Jan Smalle ([email protected])


Journal club format

Journal club format

Why?

Practice how to prepare papers and express ideas correctly.

Training in science debate.

Practice presentation: research seminar.


Targeted protein degradation

TARGETED PROTEIN DEGRADATION

Lectures: January 15, 17

Exam: January 22


Pls 623 physiology of plants ii spring semester 2014

Central dogma of genetics


Pls 623 physiology of plants ii spring semester 2014

Central dogma of genetics

Regulation of an organisms differentiation and responses to the environment are the result of changes in gene expression.

OLD THEORY: Regulation of gene expression is predominantly controlled at the transcription level.

NEW THEORY: Regulation of gene expression is the result of a combination of protein synthesis (transcription/translation) and protein degradation controls.


Pls 623 physiology of plants ii spring semester 2014

EXAMPLE

Day/night cycle: day light activates gene expression

Light

PROTEIN

DNA

RNA

Light responses


Pls 623 physiology of plants ii spring semester 2014

Dark

DNA

RNA

PROTEIN ????

Light responses ?

Day/night cycle: During night time, light-inducible genes are down-regulated

However, regulatory proteins that promote the light response need to be removed in the dark. Otherwise, they would interfere with the dark response.


Photomorphogenesis

Photomorphogenesis

HY5: a transcriptional activator of

photomorphogenic

development

Light intensity

5 days old Arabidopsis seedlings grown under a range of light intensities

Western blot using HY5 antiserum

(Osterlund et al., 2000)


Ethylene signaling

Ethylene signaling

EIN3: a transcriptional activator of

ethylene responses

Western blot using EIN3 antiserum

4 days old Arabidopsis seedlings grown in the dark on a range of concentrations of the ethylene precursor ACC

(Guo and Ecker, 2003)


Pls 623 physiology of plants ii spring semester 2014

PRO +

PROTEIN

Q

O

O

O

I

I

I

I

P

P

P

P

T

T

T

T

N

N

N

N

R

R

R

R

E

E

E

E

Proteolysis

Errors:

Misfolded:

Cleaved:

AA supply:

Regulation:

T

PRQTEIN

PRO

PROPROTEIN

PROTEIN

PROTEIN

EIN

PROTEIN


Non specific proteolysis

Non-specific Proteolysis

PROTEIN

PROTEIN

PROTEIN

PROTEIN

PROTEIN

PROTEIN

O

O

O

O

O

O

I

I

I

I

I

I

P

P

P

P

P

P

T

T

T

T

T

T

N

N

N

N

N

N

R

R

R

R

R

R

E

E

E

E

E

E


Specific or targeted proteolysis

Specific or Targeted Proteolysis

PROTEIN

O

I

P

T

N

R

E

PROTEIN

PROTEIN

PROTEIN

PROTEIN

PROTEIN


Conditional proteolysis

Conditional Proteolysis

PROTEIN

O

I

P

T

N

R

E

DARK

PROTEIN

PROTEIN

PROTEIN

PROTEIN

PROTEIN


Conditional proteolysis1

Conditional Proteolysis

O

I

P

T

N

R

E

Many cases of conditional proteolysis involve secondary modifications of target proteins.

For example: phosphorylation/dephosphorylation.

*

Kinase

PROTEIN

PROTEIN

Phosphatase


Conditional proteolysis2

Conditional Proteolysis

O

I

P

T

N

R

E

Many cases of conditional proteolysis involve secondary modifications of target proteins.

For example: phosphorylation/dephosphorylation.

*

Kinase

PROTEIN

PROTEIN

Phosphatase


Conditional proteolysis3

Conditional Proteolysis

O

I

P

T

N

R

E

Signal

Receptor

X?

*

PROTEIN

PROTEIN

PROTEIN

PROTEIN

PROTEIN

PROTEIN


Pls 623 physiology of plants ii spring semester 2014

[Vierstra, 2003 TIPS]


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