Download
1 / 20

Clinical Microbiology - PowerPoint PPT Presentation


  • 100 Views
  • Uploaded on

Clinical Microbiology. (MLCM- 201) Prof. Dr. Ebtisam. F. El Ghazzawi. Medical Research Institute (MRI) Alexandria University. Intended Learning Outcomes.

loader
I am the owner, or an agent authorized to act on behalf of the owner, of the copyrighted work described.
capcha
Download Presentation

PowerPoint Slideshow about ' Clinical Microbiology' - wesley-doyle


An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -
Presentation Transcript
Clinical microbiology

Clinical Microbiology

(MLCM- 201)

Prof. Dr. Ebtisam. F. El Ghazzawi.

Medical Research Institute (MRI)

Alexandria University


Intended learning outcomes
Intended Learning Outcomes

  • By the end of this lecture the student should be able to understand all about Pathogens Primary within the Enteric Tract, Types, pathogens, and there Laboratory Diagnostic tests.



Shigella:

Shigella species cause enterocolitis. Enterocolitis caused by Shigella is often called bacillary dysentery. The term dysentery refers to bloody diarrhea. Shigelleosis is only a human disease, its natural habitat is limited to the intestinal tract. The organism is transmitted by the oral-fecal route.


The four Fs-fingers, flies, food and feces – are the principal factors in transmission. After a short incubation period (1-2 days), there is a sudden onset of abdominal pain, fever, and watery diarrhea. The diarrhea has been attributed to an exotoxin acting in the small intestine.

A day or so later, as the infection involves the ileum and colon, the number of stools increases, they are less liquid but often contain mucus and blood.


Each bowel movement is principal factors in transmission. After a short incubation period (1-2 days), there is a sudden onset of abdominal pain, fever, and watery diarrhea. The diarrhea has been attributed to an accompaning by straining and tenesmus (rectal spasms) which resulting lower abdominal pain.

In adult cases fever and diarrhea subside spontaneously in 2-5 days.

However in children and the elderly, loss of water and electrolytes may lead to dehydration, acidosis and even death.


Laboratory diagnosis: principal factors in transmission. After a short incubation period (1-2 days), there is a sudden onset of abdominal pain, fever, and watery diarrhea. The diarrhea has been attributed to an

Specimens: Include fresh stool, mucus flecks, and rectal swabs for culture. Large numbers of fecal leukocytes and some red blood cells often are seen microscopically.

Culture: Culturing on differential media e.g. Mac-Conkey’s or EMB agar and on selective media e.g. Salmonella Shigella agar (SS agar) gives colourless colonies (NLF) should be subjected to biochemical examination.


Serology: principal factors in transmission. After a short incubation period (1-2 days), there is a sudden onset of abdominal pain, fever, and watery diarrhea. The diarrhea has been attributed to an Normal persons often have agglutinins against several Shigella species. However, serial determinations of antibody titers may show a rise in specific anitbody.


Vibrios principal factors in transmission. After a short incubation period (1-2 days), there is a sudden onset of abdominal pain, fever, and watery diarrhea. The diarrhea has been attributed to an

  • The Vibrios are curved, comma-shaped gram-negative motile rods. They are found in marine and surface waters.

Vibriocholerae

Gram stain


The major pathogen in the genus principal factors in transmission. After a short incubation period (1-2 days), there is a sudden onset of abdominal pain, fever, and watery diarrhea. The diarrhea has been attributed to an Vibrio is the Vibriocholerae which is the cause of cholera. While Vibrioparahaemolyticus causes diarrhea associated with eating raw or improperly cooked seafood.


I. principal factors in transmission. After a short incubation period (1-2 days), there is a sudden onset of abdominal pain, fever, and watery diarrhea. The diarrhea has been attributed to an Vibrio cholera:

V. cholera is transmitted by fecal contamination of water and food primarily from human sources.

A major epidemic of cholera spanned the 1960s,1970s, 1991 and 1992 had spread to many countries all over the world.

The factors that predispose to epidemics are poor sanitation, malnutrition, overcrowding and inadequate medical services.


Vibrio principal factors in transmission. After a short incubation period (1-2 days), there is a sudden onset of abdominal pain, fever, and watery diarrhea. The diarrhea has been attributed to an cholera produces an enterotoxin that causes cholera, a profuse watery diarrhea that can rapidly lead to dehydration and death.

There are no red blood cells or white blood cells in the stool.

Rice water stool is the term often applied to the nonbloody effluent.

There is no abdominal pain and subsequent symptoms are referable to the marked dehydration.


The loss of fluid and electrolytes leads to cardiac and renal failure.

Acidosis and hypokalemia also occur as a result of loss of bicarbonate and potassium in the stool.

Laboratory diagnosis:

Culture of the diarrhea stool show colourless colonies on Mac Conkey’s agar.

The organism is oxidase positive, ferment sucrose and mannose. It grows on media containing 6% NaCl.


II. renal failure.Vibrioparahaemolyticus:

Is a marine organism transmitted by ingestion of raw or undercooked seafood, especially shellfish such as oysters. Vibrioparahaemolyticus secrets enterotoxin similar to that of cholera and its clinical picture varies from mild to quite severe watery diarrhea, nausea, vomiting, abdominal cramps and fever.


The enteritis tends to subsides spontaneously in 1-4 days with no treatment other than restoration of water and electrolyte balance.

It grows in 8% NaCl solution.

It grows on TCBS agar where it yields green colonies. It is oxidase positive and grows on blood agar.


Assignment
Assignment with no treatment other than restoration of water and electrolyte balance.

  • Campylobacter,Helicobacter


Questions
Questions with no treatment other than restoration of water and electrolyte balance.

  • ……………species cause enterocolitis.

  • The term dysentery refers to…………….

  • The …………….are curved, comma-shaped gram-negative motile rods.

  • The major pathogen in the genus Vibrio is the ……………..which is the cause of …………..


Recommended textbooks
Recommended Textbooks with no treatment other than restoration of water and electrolyte balance.

  • Manual of Clinical Microbiology, Vols. 1 and 2: Eighth Edition Patrick R. Murray


ad