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How can HTRF ® fulfill your needs for Bioprocess applications?

How can HTRF ® fulfill your needs for Bioprocess applications?. April 2013. A comprehensive line of reagents that matches your needs. Antibody screening and quantification. Tagged-protein detection. Toolbox reagents to build up your screening assay .

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How can HTRF ® fulfill your needs for Bioprocess applications?

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  1. How can HTRF®fulfill your needs for Bioprocess applications? April 2013

  2. A comprehensive line of reagents that matches your needs Antibody screening and quantification Tagged-protein detection Toolbox reagents to build up your screening assay Cisbio Services to get your specific Bioprocess assay developed HTRF® solutions for bioprocess

  3. Ready to use solutions April 2013

  4. Quantify your antibody sample with ready-to-use kits Features: Compatible with a variety of biological materials A single 2-hour, room temperature incubation Detection limit: 5 ng/ml, working range up to 4,000 ng/ml Specificity: Human subclasses IgG1, IgG2, IgG3 and IgG4 The humanFckit HTRF® solutions for bioprocess

  5. Quantify your antibody sample with ready-to-use kits Features: Selective for human κ and λ IgG light chains Detection of human IgG1, IgG2, IgG3 and IgG4 No cross-reactivity with mouse IgG or human IgM Detection limit at 0.4 ng/mL and working range up to 200 ng/mL Human κ and λ kits HTRF® solutions for bioprocess

  6. Check for tag accessibility For detecting and measuring all GST and 6HIS tagged proteins during production processes Immuno-competition formats based on specific 6HIS or GST antibodies One single-step incubation GST and 6HIS check kits HTRF® solutions for bioprocess

  7. CHO HCP Anti-CHO HCP Cryptate conjugate Anti-CHO HCP d2 conjugate CHO Host CellProteindetectionassay (CHO HCP) This assay is designed for the quantitative determination of CHO HCP. CHO HCP is measured in a sandwich assay format using 2 specific antibodies, one labelled with Cryptate (donor) and the second with d2 (acceptor). The intensity of the signal obtained is proportional to the number of antigen-antibody complexes formed and therefore the fluorescence is proportional to the concentration of the antigen. HTRF® solutions for bioprocess

  8. Do It Yourself solutions April 2013

  9. Pick & choose your toolbox reagents to build up your Bioprocess assay Anti-Tag Reagents Europium Terbium XL665d2Anti-GST(GSS11)  Anti-6HIS (HIS-1)  Anti-c-myc (9E10)  Anti-FLAG (M2)  Anti-HA (HAS01)  Anti MBP  Anti-DNP  Anti RAGE - Cryptate SA-XL665 Biot-hRAGE Anti-ImmunoglobulinReagents Europium XL665 Anti-human Ig (&d2) Anti-mouse Ig (&d2) Anti-rabbit Ig Protein A  Labeling kits EuropiumLumi4®-Terbiumd2 AffinityReagents Europium XL665 Streptavidin (&Tb)  (&d2) SA-Xlent!  HTRF® solutions for bioprocess

  10. Case study: assay set up to measure RAGE / humanized anti-RAGE interaction Label the anti-RAGE antibody using the cryptatelabelingkit Combine with SA-XL665 toolbox reagent Anti RAGE - Cryptate SA-XL665 Biot-hRAGE • Features: • Antibody labeling process completed within 1 hour • Immuno-competition format for scFv affinity maturation • Great assay development flexibility • Finlay WJ. &t all. Affinity maturation of a Humanized rat antibody for anti-RAGE Therapy: comprehensive mutagenesis reveals a high level of mutational plasticity both inside and outside the complementary-determining regions. J.Mol. Biol 2009. 38, 541-558. HTRF® solutions for bioprocess

  11. Cisbio services April 2013

  12. Looking for other assays? Transform yourELISAs into a truly homogeneous high throughput method: let us do it for you! • A team of over 20 highly experienced scientists that supports and customizes your assays • A multidisciplinary approach that covers all optimization steps along the assay development process • Fast turn-around time and cost-efficient solutions HTRF® solutions for bioprocess

  13. Best pair / full AD: select your preferred option Best pair package Start your assay development with the optimal reagents Full assay development package Customized reagents and assay optimization to meet your specific needs Full assay development Best pair Assay feasibility study In collaboration with your scientific consultant 1 • Assay development • - Reagent labeling • Best pair choice • Full optimization 2 Technological transfer With scientific consultant 3 Follow-up on site 4 HTRF® solutions for bioprocess

  14. All in one wellNo-wash Flexibility Get your assay developed! • Abandon tedious assay protocols for mix & measure assays: Dispense  Incubate  Read • Broad range of conjugated bioprobes for an unlimited range of assay possibilities • Have your specific assay developed to your specifications by Cisbio Services HTRF® solutions for bioprocess

  15. Thank you

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