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HBL100. 1.2. sh-Control. sh-Control. sh-Rictor. sh-Rictor. 1.0. sh-Raptor. sh-Raptor. sh-mTOR. sh-mTOR. 0.8. MTT Metabolization (A 570 ). 0.6. 0.4. 0.2. 0. 0. 1. 2. 3. Days. A. sh-Control. sh-Rictor. sh-Raptor. sh-mTOR. sh-mTOR. sh-Control. sh-Raptor. sh-Rictor.

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Sh control

HBL100

1.2

sh-Control

sh-Control

sh-Rictor

sh-Rictor

1.0

sh-Raptor

sh-Raptor

sh-mTOR

sh-mTOR

0.8

MTT Metabolization (A570)

0.6

0.4

0.2

0

0

1

2

3

Days

A

sh-Control

sh-Rictor

sh-Raptor

sh-mTOR

sh-mTOR

sh-Control

sh-Raptor

sh-Rictor

Raptor

Raptor

Rictor

Rictor

mTOR

mTOR

GAPDH

GAPDH

MDA-MB231

HBL100

B

MDA-MB231

1.4

1.2

1.0

0.8

MTT Metabolization (A570)

0.6

0.4

0.2

0

0

1

2

3

Days

Supplementary Figure 2. A, Knockdown of different mTORC components in HBL100 and MDA-MB231. The amount of the different proteins was analysed by Western blot. B, Effect of the knockdown of mTOR, Raptor and Rictor on the proliferation of HBL100 and MDA-MB231. Cells were infected with viruses which included shRNAs for mTOR, Raptor or Rictor, selected with puromycin for 48 hours, and MTT metabolization was measured at the indicated times. Results are plotted as the mean ± SD of an experiment that was repeated twice with similar results. These data were obtained with shRNAs distinct from those used in Figure 2.

Supplementary Figure 2


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