1 / 12

Teknik & Aplikasi Kuljar Dalam Produksi & Kualitas Tanaman

Teknik & Aplikasi Kuljar Dalam Produksi & Kualitas Tanaman. “… the art and science of multiplying plants in vitro .”. Dr. Sukendah , MSc. Pembentukan Benih Sintetik.

wauna
Download Presentation

Teknik & Aplikasi Kuljar Dalam Produksi & Kualitas Tanaman

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Teknik & AplikasiKuljar DalamProduksi & KualitasTanaman “… the art and science of multiplying plants in vitro.” Dr. Sukendah, MSc

  2. PembentukanBenihSintetik Benihsintetik (Synthetic/Artificial Seed) adalahproduksibenihdariembriosomatik yang dibungkusdalam mantel tiruan Artificial seed coat Somatic embryo Artificial endosperm

  3. TeknikPembuatanBenihSintetik • MS medium + phytohormone + carbon sources. • Sodium alginate was added to the medium and mixed well to make a homogenous solution. • The explants were taken and dipped into the alginate solution. • Explants dipped in alginate solution were taken up by a forcep and placed to the beaker of CaCl2 • Each explant then became a hard ball encoded by alginate.

  4. TeknikPembuatanBenihSintetik BahanBenih: EmbrioSomatik Seed 3% Sodium alginate + MS free calcium salt solution Sodium alginate Calcium cloride Pembentukanseedcoat Seed Coat 75 mMCaCl2.2H2O Artificial Seed

  5. Haploid Plant Production • Embryo rescue of interspecific crosses • Creation of alloploids (e.g. triticale) • Bulbosum method • Anther culture/Microspore culture • Culturing of Anthers or Pollen grains (microspores) • Derive a mature plant from a single microspore • Ovule culture • Culturing of unfertilized ovules (macrospores) • Sometimes “trick” ovule into thinking it has been fertilized

  6. Anther/Microspore Culture

  7. Anther/Microspore Culture Factors • Genotype • As with all tissue culture techniques • Growth of mother plant • Usually requires optimum growing conditions • Correct stage of pollen development • Need to be able to switch pollen development from gametogenesis to embryogenesis • Pretreatment of anthers • Cold or heat have both been effective • Culture media • Additives, Agar vs. ‘Floating’

  8. What do you do with the haploid? • Weak, sterile plant • Usually want to double the chromosomes, creating a dihaploid plant with normal growth & fertility • Chromosomes can be doubled by • Colchicine treatment • Spontaneous doubling • Tends to occur in all haploids at varying levels • Many systems rely on it, using visual observation to detect spontaneous dihaploids • Can be confirmed using flow cytometry

  9. Protoplast Created by degrading the cell wall using enzymes

  10. Protoplast fusion • Protoplasts are made from two species that you want to cross • The membranes are made to fuse • osmotic shock, electrical current, virus • Regenerate the hybrid fusion product • Contain genome from both organisms • Very, very difficult

  11. Thank You

More Related