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A. 140. CCN3/NOV. 120. 100. 80. rel. mRNA expression. 60. C. 40. M. KC. LSEC. pMF. HSC. Hepatocytes. 20. 0. 2d. 2d. 0d. 0d. sec. 0d. 2d. 3d. 0d. 1d. 2d. 3d. 1d. 3d. 5d. 7d. 8d. 2d. 4d. HSC. MFB. CCN3/NOV (40 cycles). 4.5. CCN2/CTGF. 4. 3.5. GAPDH. 3.

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LSEC

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Lsec

A

140

CCN3/NOV

120

100

80

rel. mRNA expression

60

C

40

M

KC

LSEC

pMF

HSC

Hepatocytes

20

0

2d

2d

0d

0d

sec.

0d

2d

3d

0d

1d

2d

3d

1d

3d

5d

7d

8d

2d

4d

HSC

MFB

CCN3/NOV

(40 cycles)

4.5

CCN2/CTGF

4

3.5

GAPDH

3

2.5

rel. mRNA expression

2

1.5

D

1

0.5

0

1d

3d

5d

7d

8d

2d

4d

HSC

MFB

7.0

-SMA

6.0

5.0

4.0

rel. mRNA expression

CCN3/NOV (green)

ED2 (red)

3.0

2.0

1.0

0

1d

3d

5d

7d

8d

2d

4d

HSC

MFB

B

p = 0.02

35

CCN2/CTGF

30

p = 0.04

merge

DAPI (blue)

25

20

relative mRNA expression

15

10

5

0

0d

1d

2d

3d

Hepatocyte


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