Bed Nucleus of the Stria Terminalis Subregions Differentially Regulate Hypothalmic-Pituitary-Adrenal...
Download
1 / 27

Introduction - PowerPoint PPT Presentation


  • 74 Views
  • Uploaded on

Bed Nucleus of the Stria Terminalis Subregions Differentially Regulate Hypothalmic-Pituitary-Adrenal Axis Activity: Implications for the Integration of Limbic Inputs. Dennis C. Choi, Amy R. Furay, Nathan K. Evanson, Michelle M. Ostrander, Yvonne M. Ulrich-Lai, and James P. Herman. Introduction.

loader
I am the owner, or an agent authorized to act on behalf of the owner, of the copyrighted work described.
capcha
Download Presentation

PowerPoint Slideshow about ' Introduction' - violet


An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -
Presentation Transcript

Bed Nucleus of the Stria Terminalis Subregions Differentially Regulate Hypothalmic-Pituitary-Adrenal Axis Activity: Implications for the Integration of Limbic Inputs

Dennis C. Choi, Amy R. Furay, Nathan K. Evanson, Michelle M. Ostrander, Yvonne M. Ulrich-Lai, and James P. Herman


Introduction
Introduction Differentially Regulate Hypothalmic-Pituitary-Adrenal Axis Activity: Implications for the Integration of Limbic Inputs

  • Amygdala, hippocampus, and medial prefrontal cortex influence HPA axis

  • LimbicBNSTPVN

  • BNST subdivided


The big point
The Big Point Differentially Regulate Hypothalmic-Pituitary-Adrenal Axis Activity: Implications for the Integration of Limbic Inputs

  • Subregions of the BNST differentially regulate HPA axis responses to acute stress


Nijsen paper background information
Nijsen Paper: background information Differentially Regulate Hypothalmic-Pituitary-Adrenal Axis Activity: Implications for the Integration of Limbic Inputs

  • BNST is involved in autonomic and behavioral reactions

  • BNST is a rostral forebrain structure

  • Enclosed by lateral ventricle, lateral septum, fornix, nucleus accumbens, preoptic area, and hypothalamus

  • Links amygdala and hippocampus with PVN and brain stem


Methods and materials
Methods and Materials Differentially Regulate Hypothalmic-Pituitary-Adrenal Axis Activity: Implications for the Integration of Limbic Inputs

  • 36 male Sprague Dawley rats (275-300g)

  • 3/cage

  • Food/water ad libitum

  • Temp and humidity controlled

  • 12h light/dark (Lights on at 6am)


Methods and materials1
Methods and Materials Differentially Regulate Hypothalmic-Pituitary-Adrenal Axis Activity: Implications for the Integration of Limbic Inputs

  • Day 1: Surgery

  • Days 2-8: Recovery

  • Day 8: Restraint stress (9:30-10:30am)

  • Day 9-15: Nonhandled recovery

  • Day 16: 2nd restraint stress and killed

    • Killed 30 min after restraint for peak of c-fos


Methods and materials2
Methods and Materials Differentially Regulate Hypothalmic-Pituitary-Adrenal Axis Activity: Implications for the Integration of Limbic Inputs

  • Lesions identified

    • Needle track

    • Loss of neurons

    • Gliosis (increase in size and number of astrocytes)


Animals included
Animals included Differentially Regulate Hypothalmic-Pituitary-Adrenal Axis Activity: Implications for the Integration of Limbic Inputs

  • Bilateral damage included

  • Partial unilateral lesions removed

  • Missed lesions removed


Results
Results Differentially Regulate Hypothalmic-Pituitary-Adrenal Axis Activity: Implications for the Integration of Limbic Inputs

  • Anterior BNST lesions damage fusiform and dorsomedial nuclei

  • Posterior BNST lesions damage principle, interfascicular, and transverse nuclei


C fos crh gad 65 mrna expression in bnst
C-fos, CRH, GAD 65 Differentially Regulate Hypothalmic-Pituitary-Adrenal Axis Activity: Implications for the Integration of Limbic InputsmRNA expression in BNST

  • C-fos decreased with anterior lesion

  • CRF decreased with anterior lesion

  • GAD 65 decreased with anterior lesion

  • GAD 65 diminished with posterior lesion

    • \ lesions were successful


Successful lesions
Successful Lesions Differentially Regulate Hypothalmic-Pituitary-Adrenal Axis Activity: Implications for the Integration of Limbic Inputs


C fos mrna expression
C-Fos mRNA expression Differentially Regulate Hypothalmic-Pituitary-Adrenal Axis Activity: Implications for the Integration of Limbic Inputs


Plasma acth
Plasma ACTH Differentially Regulate Hypothalmic-Pituitary-Adrenal Axis Activity: Implications for the Integration of Limbic Inputs

  • Elevated at 30 and 60 min (all groups)

  • Posterior lesion elevated

  • Anterior lesion no effect

  • Same at 0 or 120 min (all)


Plasma acth graph
Plasma ACTH graph Differentially Regulate Hypothalmic-Pituitary-Adrenal Axis Activity: Implications for the Integration of Limbic Inputs


Plasma corticosterone
Plasma Corticosterone Differentially Regulate Hypothalmic-Pituitary-Adrenal Axis Activity: Implications for the Integration of Limbic Inputs

  • Increased B to restraint

  • Anterior lesion decreased B at 30 min

  • Posterior lesion increased B at 60 min


Plasma cort graph
Plasma Cort graph Differentially Regulate Hypothalmic-Pituitary-Adrenal Axis Activity: Implications for the Integration of Limbic Inputs


Posterior bnst lesion and the hpa axis
Posterior BNST lesion and the HPA axis Differentially Regulate Hypothalmic-Pituitary-Adrenal Axis Activity: Implications for the Integration of Limbic Inputs

  • Increased secretion of ACTH and B

  • Increased c-fos mRNA

  • Increased CRH and AVP mRNA in PVN

  • Inhibits HPA response to stress


Posterior bnst
Posterior BNST Differentially Regulate Hypothalmic-Pituitary-Adrenal Axis Activity: Implications for the Integration of Limbic Inputs

  • Restraint induced c-fos expression enhanced by lesion

  • Principal nucleus inhibits the PVNmp

  • Principal nucleus regulates HPA activity


Anterior bnst lesion and the hpa axis
Anterior BNST lesion and the HPA axis Differentially Regulate Hypothalmic-Pituitary-Adrenal Axis Activity: Implications for the Integration of Limbic Inputs

  • Decrease B and c-fos

  • No alteration of CRH and AVP in PVN

  • CRH and Glu activate HPA


Discussion
Discussion Differentially Regulate Hypothalmic-Pituitary-Adrenal Axis Activity: Implications for the Integration of Limbic Inputs

  • Specific roles of BNST nuclei

  • Anterior excites PVN, promotes B secretion

  • Posterior inhibits PVN excitation, ACTH release, and B responses

  • Posterior lesion enhances c-fos expression


Mcgill paper

Crh gene expressed in BNST Differentially Regulate Hypothalmic-Pituitary-Adrenal Axis Activity: Implications for the Integration of Limbic Inputs

Encodes CRH

Increased anxiety and stress

Overexpression HPA dysfunction

McGill Paper

308/Y

WT


Nijsen paper
Nijsen Paper Differentially Regulate Hypothalmic-Pituitary-Adrenal Axis Activity: Implications for the Integration of Limbic Inputs

  • Restraint stress induces c-fos expression

  • Stress activates the CRH system in BNST

  • Not directly related to autonomic stress

  • Site of CRH determines response

  • CRH in medial BNST stress induced


Discussion1
Discussion Differentially Regulate Hypothalmic-Pituitary-Adrenal Axis Activity: Implications for the Integration of Limbic Inputs

  • BNST is a “clearing house” to regulate HPA

  • Posterior BNST inhibits HPA activity to restraint

  • Lesioning primary nuclei increased

    • ACTH and B

    • C-fos mRNA

    • CRH and AVP


The end
The End Differentially Regulate Hypothalmic-Pituitary-Adrenal Axis Activity: Implications for the Integration of Limbic Inputs


ad