1 / 30

Effects of UVB radiation on the skin lipids of garter snakes

Effects of UVB radiation on the skin lipids of garter snakes. Anna Vigeland Dr. M. Rockwell Parker Dr. Robert T. Mason. Study Species. Red-sided Garter Snake ( Thamnophis sirtalis parietalis ) Model organism Hibernate 8 months in large hibernacula Emerge by the thousands in late spring

vic
Download Presentation

Effects of UVB radiation on the skin lipids of garter snakes

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript


  1. Effects of UVB radiation on the skin lipids of garter snakes Anna Vigeland Dr. M. Rockwell Parker Dr. Robert T. Mason

  2. Study Species Red-sided Garter Snake (Thamnophis sirtalis parietalis) • Model organism • Hibernate 8 months in large hibernacula • Emerge by the thousands in late spring • Breeding follows for a few weeks • Courtship behavior mediated by pheromones produced in the skin

  3. Garter Snake Environment • Most northerly living reptile • Study snakes caught at dens in Manitoba • Northern regions strongly subject to environmental changes • UVB radiation

  4. Skin Lipid Profile • 18 long-chain saturated and monounsaturated methyl ketones in pheromone • High ratios of unsaturated and high molecular weight methyl ketones are associated with large, healthy females • Males differentiated in part by the presence of squalene more attractive less attractive

  5. Skin Lipid Profile Gas chromatograph of a typical female pheromone sample 504 Saturated Unsaturated 478 532 450 518 476 506 490 448 422 464 492 436 394 420 462 408 434

  6. Objective Methyl ketone ratios change during the summer in the field but not in the lab Why?

  7. Hypothesis Ultraviolet radiation causes qualitative changes in the skin lipid profiles of red-sided garter snakes

  8. Question 1 Does ultraviolet-B radiation have an effect on the female sexual attractiveness pheromone?

  9. Methods

  10. UVB radiation on female pheromone samples Full-spectrum light only Full-spectrum and UVB light

  11. * p < 0.05 * * * * * * *

  12. UVB exposure affects the ratios of pheromone samples p = 0.00934 p = 0.00934

  13. Question 2 Does ultraviolet-B radiation have the same effect on the female sexual attractiveness pheromone when in mixture with other skin lipids?

  14. Methods

  15. Pheromone irradiated within neat solution Full spectrum light only Full spectrum and UVB light

  16. Pheromone irradiated within neat solution

  17. Live-animal exposure: overview • Two groups of snakes exposed to either full spectrum or full spectrum +UVB light • Behavioral and physical changes examined after prolonged UV exposure • Skin lipid analysis after exposure period

  18. Question 3 Does exposure to ultraviolet-B radiation have an effect on the behavior of red-sided garter snakes?

  19. Methods Short-term: detection and/or avoidance of UVB light • Cardboard tube test: % snakes seeking refuge in tubes • Mylar filter test: % snakes situated under Mylar vs. acetate Long-term: snake activity levels • Observation and categorization of behaviors: % snakes active, alert, or resting

  20. UVB radiation does not affect snake behavior • No significant differences in number of snakes seeking refuge in cardboard tubes • Equal numbers of snakes situated under Mylar filters as under acetate filters • No significant differences in the activity levels between treatment groups or after the 15-day exposure period

  21. Question 4 What effects does daily exposure to ultraviolet-B radiation have on the neutral skin lipids of red-sided garter snakes?

  22. Methods • Skin lipid extraction by soaking in hexane • Purification of female attractiveness pheromone and squalene by alumina column chromatography • Analysis of pheromone concentrations by gas chromatography

  23. Daily UVB on live snake pheromone ratios

  24. Environmental Implications • UVB radiation has an effect on the female attractiveness pheromone and its US:S ratio • UVB radiation has been increasing, especially in polar latitudes, due to ozone depletion • Increases in UVB could affect red-sided garter snake reproductive behavior

  25. Further Research • Gas chromatography analysis of squalene concentrations • Thin layer chromatography to test for major differences in other neutral skin lipids

  26. Acknowledgements Funding provided by: • Howard Hughes Medical Institute Special thanks to: • Dr. Robert T. Mason • Dr. M. Rockwell Parker • Mason Lab: Emily Uhrig, Mattie Squire, Chris Friesen, Ben Burke • The Blaustein Lab

  27. Questions?

More Related