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Electroporation the phenomenon and technical   approach to experiments. Peter Kramar University of Ljubljana Faculty of Electrical Engineering http://lbk.fe.uni-lj.si [email protected] Wroclaw, 24.4.2009. Laboratory of Biocybernetics. http://lbk.fe.uni-lj.si. The phenomena.

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Electroporation the phenomenon and technical   approach to experiments

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Electroporationthe phenomenon and technical  approach to experiments

Peter Kramar

University of Ljubljana

Faculty of Electrical Engineering

http://lbk.fe.uni-lj.si

[email protected]

Wroclaw, 24.4.2009


Laboratory of Biocybernetics

http://lbk.fe.uni-lj.si


The phenomena


The phenomena


Applications

Reversibleelectroporation:

Cell fusion

Gene transfection

Drug delivery

Cancer treatment

Ireversible electroporation:

Food pasterization

Tissueablation


Key issue

The phenomena is already used (even in clinical applications) and it works well in several applications, but the basic principal are not clarified yet.


Levels of electroporation

  • Planar lipid bilayers

  • Lipid vesicles

  • Cells in suspension

  • Attached cells

  • Cell organelles

  • Tissue


Levels of electroporation

  • Planar lipid bilayers

  • Lipid vesicles

  • Cells in suspension

  • Attached cells

  • Cell organelles

  • Tissue


Previous experiments


Experimental systems

Voltage clamp

Kramar et al, Bioelectrochemistry 2007

Current clamp

Kalinowski et al, Meas Sci Technol1995


Measurement protocol


Membrane formation

1-palmitoyl 2-oleoyl phosphatidylcholine POPC

1-palmitoyl 2-oleoyl phosphatidylserine POPS

POPC:POPS mixtures 1:3, 1:1and 3:1

Lipids

Aqueous

solution

Rising the level of

aqueous solution

Montal M., Mueller P. PNAS69: 3561-3566, 1972


Capacitance measurement

Discharge method

Benz et al, ????

Capacitance to period converterKalinowski et al, 1995


Voltage clamp - linear rising signal

kU

Kramar et al., 2007


Current clamp - linear rising signal


http://www.cliniporator.com/ect


Thank you for your attention!


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