Amgen biotech lab series
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Amgen Biotech Lab Series. LAB 1: practice micropipetting & loading a gel. Micropipet Use. . Twist dial to desired volume . Add disposable pipet tip . Press plunger to first stop . Insert pipet tip into solution to be transferred

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Micropipet Use

  • . Twist dial to desired volume

  • . Add disposable pipet tip

  • . Press plunger to first stop

  • . Insert pipet tip into solution to be

  • transferred

  • . Slowly release plunger to retrieve liquid

  • . Move pipet tip into desired tube

  • . Press plunger past first stop to second stop to transfer liquid, keep the plunger down as you remove it from the tube.

  • 8. Eject tip



Loading a gel
Loading a gel

  • Micropipette desired amount

  • Place tip ABOVE the well in the gel

  • Push through to the 2nd stop

  • Keep plunger depressed while you pull the micropipette out of the gel box

  • Write down which well you loaded on the handout taped to the lab bench

Do NOT pierce through the bottom of the gel!



Micropipetting technique A


technique B

Close the tips!


Technique C

Billiard style





Lab 1
Lab 1

  • uses 3 different colored dye molecules that are different sizes and different charges

  • The A, B, & C lane will either be 1 type of dye or a mixture of 2 or 3 dyes.

  • What does the gel below show?

    • Lane 1: DNA ladder

    • Lane 5: 397bp vs 187bp

Animation in “Animation” File


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