Amgen biotech lab series
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Amgen Biotech Lab Series. LAB 1: practice micropipetting & loading a gel. Micropipet Use. . Twist dial to desired volume . Add disposable pipet tip . Press plunger to first stop . Insert pipet tip into solution to be transferred

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Amgen Biotech Lab Series

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Amgen biotech lab series

Amgen Biotech Lab Series


Lab 1 practice micropipetting loading a gel

LAB 1: practice micropipetting & loading a gel


Amgen biotech lab series

Micropipet Use

  • . Twist dial to desired volume

  • . Add disposable pipet tip

  • . Press plunger to first stop

  • . Insert pipet tip into solution to be

  • transferred

  • . Slowly release plunger to retrieve liquid

  • . Move pipet tip into desired tube

  • . Press plunger past first stop to second stop to transfer liquid, keep the plunger down as you remove it from the tube.

  • 8. Eject tip


Amgen biotech lab series

Hold micropipet and epitubes at eye level


Loading a gel

Loading a gel

  • Micropipette desired amount

  • Place tip ABOVE the well in the gel

  • Push through to the 2nd stop

  • Keep plunger depressed while you pull the micropipette out of the gel box

  • Write down which well you loaded on the handout taped to the lab bench

Do NOT pierce through the bottom of the gel!


Amgen biotech lab series

Micropipet tip should be ABOVE the well NOT IN IT!!!!


Amgen biotech lab series

Micropipetting technique A


Amgen biotech lab series

technique B

Close the tips!


Amgen biotech lab series

Technique C

Billiard style


Amgen biotech lab series

Micropipet tip punched right through the gel

See dye under the wells


Amgen biotech lab series

NICE!


Amgen biotech lab series

  • Gel Electrophoresis separates molecules by:

  • Size

  • charge


Lab 1

Lab 1

  • uses 3 different colored dye molecules that are different sizes and different charges

  • The A, B, & C lane will either be 1 type of dye or a mixture of 2 or 3 dyes.

  • What does the gel below show?

    • Lane 1: DNA ladder

    • Lane 5: 397bp vs 187bp

Animation in “Animation” File


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