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Amgen Biotech Lab Series. LAB 1: practice micropipetting & loading a gel. Micropipet Use. . Twist dial to desired volume . Add disposable pipet tip . Press plunger to first stop . Insert pipet tip into solution to be transferred

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Presentation Transcript
slide3

Micropipet Use

  • . Twist dial to desired volume
  • . Add disposable pipet tip
  • . Press plunger to first stop
  • . Insert pipet tip into solution to be
  • transferred
  • . Slowly release plunger to retrieve liquid
  • . Move pipet tip into desired tube
  • . Press plunger past first stop to second stop to transfer liquid, keep the plunger down as you remove it from the tube.
  • 8. Eject tip
loading a gel
Loading a gel
  • Micropipette desired amount
  • Place tip ABOVE the well in the gel
  • Push through to the 2nd stop
  • Keep plunger depressed while you pull the micropipette out of the gel box
  • Write down which well you loaded on the handout taped to the lab bench

Do NOT pierce through the bottom of the gel!

slide8

technique B

Close the tips!

slide9

Technique C

Billiard style

lab 1
Lab 1
  • uses 3 different colored dye molecules that are different sizes and different charges
  • The A, B, & C lane will either be 1 type of dye or a mixture of 2 or 3 dyes.
  • What does the gel below show?
    • Lane 1: DNA ladder
    • Lane 5: 397bp vs 187bp

Animation in “Animation” File

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