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Normoxia

Normoxia. Normoxia. Normoxia. Normoxia. Normoxia. Normoxia. Hypoxia. Hypoxia. Hypoxia. Hypoxia. Hypoxia. Hypoxia. NS. NS. NS. NS. NS. NS. NS. Figure S1. EPO. Fold compare to control. HIF-1 a Exon 2. ***. ***. Fold compare to control. *. VEGF. Glut-1.

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Normoxia

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  1. Normoxia Normoxia Normoxia Normoxia Normoxia Normoxia Hypoxia Hypoxia Hypoxia Hypoxia Hypoxia Hypoxia NS NS NS NS NS NS NS Figure S1 EPO Fold compare to control HIF-1a Exon 2 *** *** Fold compare to control * VEGF Glut-1 Fold compare to control *** ** HK2 MCT4 Littermate Controls HIF +/-

  2. Figure S2

  3. NS Figure S3 A. NS NS EPO normalized to control Control DFO Liver Kidney B. EPO normalized to control Control DFO Serum

  4. DFO DFO DFO DFR DFR DFR NS NS NS NS NS NS NS * NS NS Figure S4 A. EPO VEGF Fold compare to control Glut-1 HK2 Fold compare to control DFO DFR B. EPO VEGF Glut-1 HK2 DFO DFO DFO DFO Controls (no DFO of DFR) Treatment

  5. NS Figure S5 NS A. NS EPO normalized to control Control DFR Liver Kidney B. EPO normalized to control Control DFR Serum

  6. Figure S6

  7. Figure S7 A. Pacsin3 FGF5 Trafd1 IL17rd Con DFO Con Con Con DFO DFO DFO B. EGR-2 EGR-4 DUSP6 sh2d3c P=.07 ** Con DFR Con DFR Con DFR Con DFR

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