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SOIL MICROBIOLOGY PROGRAM @ GRIFFIN CAMPUS. 3 rd YEAR REVIEW SPRING FACULTY MEETING, MACON, GA. Mussie Y. Habteselassie Crop and Soil Sciences UGA Griffin Campus. INTRODUCTION. Came to UGA in 2008 30% teaching / 70% research appointment Outline: Research program Sample projects

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SOIL MICROBIOLOGY PROGRAM @ GRIFFIN CAMPUS

3rd YEAR REVIEW

SPRING FACULTY MEETING, MACON, GA

Mussie Y. Habteselassie

Crop and Soil Sciences

UGA Griffin Campus

slide2

INTRODUCTION

  • Came to UGA in 2008
  • 30% teaching / 70% research appointment
  • Outline:
    • Research program
    • Sample projects
    • Teaching Responsibility
slide3

RESEARCH PROGRAM

  • Environmental Soil Microbiology Program

1. The ecology of human and animal pathogens in soils

  • 2. Environmental services of soil microbial community
  • 3. Microbial water quality
slide4

Survival

Transmission to produce

1. Ecology Of Human and Animal Pathogens In Soils

Ecology of pathogens in soils

  • Use of model microorganisms
  • - Lux marked E. coli O157:H7/Salmonella spp
  • - GFP marked E. coli O157:H7/Salmonella spp
  • - Antibiotic resistant E. coli/Salmonella spp
slide5

E. coli Persistence in the Rhizosphere of Fresh Produce

Habteselassie, et al. 2010. Understanding the role of agricultural practices in potential colonization and contamination by E. coli in rhizospheres of fresh produce. JFP 73:2001-2009.

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14

3

5

C

Growth (d)

C

C

T

T

T

Control plants were not visible due to absence of E. coli O157:H7 lux.

Tracking E. coli O157:H7 Activity and Transmission on Fresh Produce

Root

E. coli O157:H7 gfp

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Pathogen Persistence and Transmission during lettuce harvest

  • Co-PI on a grant from UC, Davis in collaboration with Dr. Cannon at the UGA Center for Food Safety: $338,414
  • Two year project (Jan 2011-Dec 2012)
  • Soil organic matter accumulation on glove during harvest and its effect on pathogen persistence and transmission
  • Source tracking organic matter from soil vs lettuce
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N & C cycling

Pollutant remediation

2. Environmental Services of Soil Microorganisms

Soil microbes & their environmental services

Approach

- Molecular microbiology – targeting functional genes

- Stable isotope techniques – process characterization

slide9

A simplified Internal N cycle in soils

Denitrification

Mineralization

Nitrification

Organic N

NH4+

NO3-

Leaching

Microbial Biomass

Plants

N Cycling Under Contrasting Agricultural Management Systems

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Changes in Soil N Cycling Organisms Following Repeated Poultry Litter application

Ammonia Oxidizing Bacteria

Ammonia Oxidizing Archaea

Control Plots

Treatment Plots – Litter @ different rates

Treatment Plots – Litter @ different rates

Control Plots

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New Project On N Cycling

  • Functional and molecular diversity in nitrogen cycle enzymes under contrasting agricultural management systems.
  • USDA-AFRI Microbial Communities Program. Habteselassie, M.Y. (Co-PI):$499,894
  • 5-year project; starts in July 2011
  • Organic vs conventional farming systems
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Volatile Organic Compounds (VOC) Metabolizing organisms in Indoor Plants

  • Seed Money from the National Foliage Foundation (NFF)
  • VOCs from different sources such as carpets, computers
  • Some indoor plants efficient in removing VOCs
  • Microorganisms in the rhizosphere important players in VOCs removal
  • Survey in two homes in Athens, GA - >100 VOCs
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Isolation and Characterization

PCR

Cloning

Sequencing

Extraction, streaking & Isolation

Identification & Characterization

Priming

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…TEACHING

Natural Resources Science Major @ the Griffin Campus

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