Serum Amylase. DESCRIPTION. AMYLASE belongs to the family of glycoside hydrolase enzymes that break down starch into glucose molecules . Amylase (EC 184.108.40.206) cleave at random locations on the starch chain, ultimately yielding maltose, glucose.
Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.
AMYLASEenzyme is synthesized primarily in the pancreas and salivaryglands.
Increased enzyme levels in humans are associated with:
Uses 2-chloro-4-nitrophenyl-maltotrioside (CNPG3) substrate, which reacts with amylase and release 2-chloro-4-nitrophenol (CNP). The increase in the absorbance is measured at 405nm and at 37° temperature, is proportionate to the enzyme activity in the sample.
5CNPG3amylase 3CNP + 2CNPG2 + 3G3 +2G
When you have symptoms of a pancreatic disorder, such as severe abdominal pain, fever, loss of appetite
Serum – heparinised plasma - urine
A blood sample drawn from a vein in the arm.
Blood was collected and allowed to stand at room temperature for 1°hour to coagulate.
Serum was obtained by centrifugation for 30 minutes at 4°C.
No loss of activity within 5 days at 4-25 °C.
What does the test result mean?
In acute pancreatitis, amylase in the blood increases (often to 4-6 times higher than the highest reference value, sometimes called upper limit of normal).