1 / 10

Unknowns

Unknowns. 1st lab session. Two unknown slants One is gram+ and the other is gram -. Unknowns will be numbered Inoculate each unknown on to a Brain Heart Infusion Agar (BHIA) – subculture (working culture) 2. Inoculate each unknown on MacConkey Agar – do streaking for isolation

ursa
Download Presentation

Unknowns

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Unknowns

  2. 1st lab session Two unknown slants One is gram+ and the other is gram -. Unknowns will be numbered • Inoculate each unknown on to a Brain Heart Infusion Agar (BHIA) – subculture (working culture) 2. Inoculate each unknown on MacConkey Agar – do streaking for isolation Gram staining on each unknown Label the plates, slides, tubes with the appropriate unknown numbers. Incubate all at 37oC

  3. 2nd lab session • Examine MacConkey for growth • No growth – gram positive • Growth – gram negative • These results will confirm the gram staining results • Do catalase test only on gram positive unknown • Catalase + Staphylococcus, catalase – Streptococcus

  4. Gram negative unknown • Examine the isolated colonies on MacConkey and determine the color of the colony. • Pink – lactose fermenter • White/colorless – lactose nonfermenter • We do not need this information for gram positive.

  5. Non- fermenter • TSI slant – only one • Urea broth • Fermenter • Tryptone broth – 1 • MRVP – 2 • Citrate slant – 1 • Urea broth – 1 • Motility - 1

  6. 3rd lab session • Match the results on your unknown with the results on the tables for the known bacteria.

  7. Table 3 Table 1 Table 2 Table 4

More Related