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第七章 病毒病的生物防治 PowerPoint PPT Presentation


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第七章 病毒病的生物防治. 吴云锋. 第一节 交互保护作用. 一、概述 1. 概念 交互保护作用 (cross‐protection) : 即如果有机体已感染了一种病毒或病毒株系后,对其它病毒或病毒株系的侵害产生抑制作用。 先感染的病毒叫 保护株系 (protecting strain) ,为弱株系,致病力弱,对寄主影响小。而后侵入的病毒叫 攻击株系 (challenge strain) ,为强株系,具强致病力,严重为害寄主。 交互保护作用本质 上是病毒之间的相生相克,从而为病毒病的防治开辟了新途径。. 2. 发展过程

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第七章 病毒病的生物防治

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3019906


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1.

(crossprotection)

(protecting strain)(challenge strain)


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2.

(Mckinney..1929)

60

70L111095 Rast(1972)(Tomato Mosaic VirusToMV)MII-16Tomlinson(1978)HNO2V3

80 Moline(1983)(Tomato Aspermy Virus)


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1985HNO2N14

N14

(PRV)

(PSV)

CMVRNACMV-S5275.5%100%81.1%82.7%

S52S54TMV


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3.

1RNADNA

(TMV)(Thung..1931;Singh..etal1970)(CaMV)34-1843NVRS(Tomlinson.. etal1978)


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2.

(TEV)(PVX)(Kassanis. etal1945)

3

(PSTV)(Fernow..1967;Niblett.. etal1979;Singh.. etal1989)

4

(RRV)12(Barhey. etal1978)

(BtCTV)(Bennett..1955)(MDMV)(Paulsen.. etal1970)


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4.

()


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(1980)2ml1ml 4mol/LNaNO21mol/L(pH4.0)1mlpH4.3;1020

0.066mol/L(pH7.0)50100pH7.0

HNO2


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a. (CP)

1985Beachy

1986(TMV)1cDNA5%99%26%35


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  • TMV

  • (AlMV)

  • (CMV)

  • (TRV)

  • X(PVX)

  • Y(PVY)

  • (SMV)

    (1993)


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b. RNA

1986HarrisonCMVRNAcDNACMV

Gerlah(ToRSV)RNA


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2

1.

253-451030

70 C..


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2.

1(1-2)N14 1003N141

215(2-3)N141001000. 56005

31-310N14400


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1.

2. 201020

3.

4. 10-15


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1.

(Tomato Mosaic VirusToMV)MII-16

19711973MII-1615%6%9%75%13%48.6%20%

(Tomato Aspermy Virus)( Moline1983)


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2. (Zucchini Yellow Mosaic Virus)

Lecoq(1991)1988198914.72.240


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3.

Kosaka(1997)CMVZYMV-2Water melon MosaicVirus-2WMV-2315%

RNACMV CMV551S12TMVCMV

(1994)CMVRNACMVP192990%


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4.

(Cauliflower Mosaic VirusCaMV)

TMV

Kosaka(1994)(Soybean Mosaic VirusSMV)Aal5-Mz


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36


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TEVSCMV, 10 2013.8%30d44d100%TEVSCMV

TEVSCMV


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  • slight etch on inoculated leaves at the later stage in treating

  • herpes on inoculated leaves at the early stage in treating

  • SCMVTEV


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  • slight etch on inoculated leaves at the later stage in treating

  • herpes on inoculated leaves

  • at the early stage in treating


  • 3019906

  • mild mosaic on inoculated leaves

  • at the later stage in treating

  • no symptom on leaves at

    The early stage in treating

    of CK without SCMV inoculation


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    CK2

    mosaic leaves at the early stage

    in CK2 without SCMV inoculation

    • CK2

  • etch on inoculated leaves at the later stage in CK2 without SCMV inoculation


  • 3019906

    A

    two rows of maizes around tobaccos in treating A

    A

    one leave of the tobacco in treating A


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    B

    five rows of tobaccos and one row of maizes in treating B


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    two rows of tobaccos around CK

    one leave of the tobacco around CK


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    1.

    1926 Mulvania ,(tobacco mosaic virus ,TMV)

    ( Pectobacterium carotovora)

    ( Pantoea stewartii )

    ( Bacillus subtilis)

    ( Aerobacter aerogens)

    (Photosyntheic bacterium ,PSB) (cucumber mosaic virus ,CMV)

    ,,


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    2.

    ,

    • TMV ;

    • (abomycin) TMV

    • Apiocrea sp. (peptaibol) peptavirins A peptavirins B TMV

    • ( Streptomyces microflavus) D2Fattiviracins ,DNA RNA


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    • ( Streptomyces noursei var. xichangensis) (ningnanmycin)TMV ,1997

    • 2001

    • ( Nocardia formica) (noformycin) TMV ,(SBMV) TMV ;

    • ( S . lavendulae) (holomycin) TMV ;

    • B (tormycin B) (gentamycin) (povamycin) (dauno2mycin)


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    ,,,

    • 1963 ,TMV

    • B25 ASA ,TMVCMV

    • (cycloheximide)

    • ( S . Guogeroffi) ( S . loivochromogenes) ( S . ramous) ( S . rochei )(ToMV) TMV


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    3.

    50 ( Rhizopus nigruicans ) ( Penicilliumgodlewskii) ( Trichoderma harzianum) ( Aspergillus candidus ) ( Neurospora sitophila) ( Colletotrichum circinans) ( Sclerotinia f ructicola) ( Gibberella saubinetii) ( Rhizoctonia solani) (TNV) TMV SBMV

    ,( Fusarium oxysporum f . sp. lycopersici) (CAVF) ; ( Trichothecium roseum) (Trichothecin)

    ,(mushroom RNA) ,( Lentinus edodes) Rentemin TMV ,3 ,15 015 %,6213 %7716 % ,15 %


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    ,,TMV79.17 %89.14 %,( Pleurotus eryngii ) ,,TMV ( Coprinus comatus) ( Pleurotus citrinopileatus ) ( Pleurotus ostreatus)( Flammulina velutipes) 20 TMV AM


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    1.

    TMV 100g/ ml 30min ,76.19 %;45min , TMV ,83.11 % ( Penicillium) TMV CMV ,1251000mg/ LSBMV 44.14 %91.13 %; TMV .


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    3716 %7412 %y3 TMV 210g/ ml,,,,,,

    ,(citromycin) ,,


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    • 2.

    • y3TMV ,TMV ,y3 ,,,; y3 ,RNase , AAVP TMV ,,AAVP ,,Retenmin RNA mRNA ,,( Endothia parasitica) skyrin (rice dwarf virus ,RDV) ,RNA


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    • 3.

    • (pathogenesis2related proteins ,PR) 88D TMV ,,PR ,PR 7 PR ,,PR 7 PR ,PR ,,,1g/ ml A ,1,3,TMV () ,A


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    • (SA) 7262PR ,; SA PRPR21 (SAR) ,TMV PGPR ,PGPR () ,PGPR ,PGPR CMV (whitefly transmitted tomato mottle virus ,ToMoV) PGPR ,


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    • ,,


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    1.

    TMV ,,,,,,,,,,,


    3019906

    2.

    ,,,,TMV ,TMV RNA ,,25 24h ,,,,,,,


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    ,,,,,1) ;2) ;3)


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    • RNA


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