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Legends PowerPoint PPT Presentation


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Legends. For ETD data In the spectra v = precursor ions, charge-reduced ones too; * = charge-reduced ion from a coeluting precursor ion with different, usually (2+), charge; m = neutral sugar losses In the sequences residue in bold = corresponding z . or z+1 ion detected;

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Legends

Legends

For ETD data

In the spectra

  • v = precursor ions, charge-reduced ones too;

  • * = charge-reduced ion from a coeluting precursor ion with different, usually (2+), charge;

  • m = neutral sugar losses

    In the sequences

  • residue in bold = corresponding z. or z+1 ion detected;

  • residue underlined = corresponding c or c-1. ion detected;

    For both HCD and ETD data

    The Table following the spectra contains all the masses used in the database search (Protein Prospector uses the 20 most abundant ions from each half of the spectra) as well as their assignments


Legends

Apolipoprotein E

Q03247

Thr-31 (& Thr-32)

v

817.0358(3+)

m

DM(Oxidation)EGELGPEEPLT(HexNAc)T(HexNAc)QQPR


Legends

Apolipoprotein E

Q03247

Thr-307

v

541.2650(3+)

v

v

m

*

LRPS(HexNAc)PTSPPSENH


Legends

Insulin-like growth factor-binding protein 5

Q05717

Thr-171

v

486.9032(3+)

v

*

m

v

FVGGAENT(HexNAc)AHPR


Legends

Collagen alpha-1(XI) chain (Fragment)

Q28083

Thr-86

v

859.8793(2+)

v

NYGTESYQT(HexNAc)EAPR


Legends

Coagulation factor V

Q28107

Ser-1151 & Thr-1154

v

759.0452(3+)

v

*

m

RS(HexNAc)PPT(HexNAc)QPSQIPNYDLR


Legends

Coagulation factor V

Q28107

Thr-1171

HCD

860.4367(2+)

v

ETD

c13

z11

c9*

c12

v

c8*

c11

b10

m

c7*

z9*

z10

AIPT(HexNAc)DVSQIFPSLE


Legends

The glycopeptide was identified from HCD data with a HexNAc neutral loss, as presented below,

mass differences are in ppm.

Site assignment from ETD as presented above.


Legends

Complement component C7

Q29RQ1

Thr-696

HCD

428.2453(3+)

EAPLTPKVPK+HexNAc


Legends

The glycopeptide was identified from HCD data with a HexNAc neutral loss, as presented below,

mass differences are in ppm.

Single potential site.


Legends

Augurin

Q32KM8

Thr-47

v

943.9625(2+)

v

m

EAPAPT(HexNAc)MTPVAVQESR


Legends

Complement factor I

Q32PI4

Thr-57

v

747.8566(4+)

v

ETEASSEVKPT(HexNAc)STQDTSQKDFVDKK


Legends

Complement component C9

Q3MHN2

Thr-24 & Ser-26

v

911.7614(3+)

v

m

*

GPT(HexNAc)PS(HexNAc)YDPAERQGTPLPIDC(Carbamidomethyl)R


Legends

Thrombospondin-4

Q3SWW8

Thr-270 & Ser-282 & Thr-284

v

793.1561(4+)

v

m

m

FQSPT(HexNAc)PNTLMPVVPAAS(HexNAc)PT(HexNAc)PPVRR


Legends

Apolipoprotein C-IV

Q3SYR5

Thr-35

1010.9789(2+)

HCD

v

ETD

y8

y13

y10

z15*

z16

m

y15

z8*

y11

m

*

y14

Q(Gln->pyro-Glu)QEEPEGT(HexNAc)LSPQPAPAR


Legends

The glycopeptide was identified from HCD data with a HexNAc neutral loss, as presented below,

mass differences are in ppm.

Site assignment from ETD as presented above.


Legends

Inter-alpha-trypsin inhibitor heavy chain H4

Q3T052

Q5EA67

Ser-629 & Ser-635

v

566.2913 (4+)

v

v

FGHS(HexNAc)VGDRTS(HexNAc)RKPGGGLK


Legends

Inter-alpha-trypsin inhibitor heavy chain H4

Q3T052

Q5EA67

Ser-677 (& Ser-683; & Ser-686 or Thr-688)

v

v

m

1078.4978(3+)

LMS(HexNAc)PLAPAS(HexNAc)APS(HexNAc)PTSGPGGASHDTDFR


Legends

Different colors indicate ambiguity about the site assignment: green supports Ser-686;

blue indicates sugar on Thr-688.


Legends

Inter-alpha-trypsin inhibitor heavy chain H4

Q3T052

Q5EA67

(Ser-683) & Ser-686 & Thr-688 & Ser-689

v

v

909.7225(3+)

m

*

AS(HexNAc)APS(HexNAc)PT(HexNAc)S(HexNAc)GPGGASHDTDFR


Legends

Inter-alpha-trypsin inhibitor heavy chain H4

Q3T052

Q5EA67

(Thr-688 or Ser-689) & Ser-695

v

604.2606(3+)

v

m

*

T(HexNAc)SGPGGAS(HexNAc)HDTDFR


Legends

Different colors indicate ambiguity about the site assignment: green supports Thr-688;

blue indicates sugar on Ser-689.


Legends

Inter-alpha-trypsin inhibitor heavy chain H4

Q3T052

Q5EA67

(Thr-688) & Thr-698

v

604.2604(3+)

m

v

*

T(HexNAc)SGPGGASHDT(HexNAc)DFR


Legends

Vitronectin

Q3ZBS7

Thr-63

v

570.6102(3+)

*

m

v

m

AEC(Carbamidomethyl)KPQVT(HexNAc)RGDVF


Legends

Both ends non-tryptic!

Precursor mass error = -0.15 ppm

Score = 26.3; E = 2.9e-4


Legends

Vitronectin

Q3ZBS7

Thr-97 & Thr-98

v

845.4210(2+)

m

*

v

QPEST(HexNAc)T(HexNAc)LAPVLQ


Legends

both ends non-tryptic!

Precursor mass error = -4.8 ppm

Score = 28.6; E = 4.5e-4


Legends

Vitronectin

Q3ZBS7

Thr-107

v

680.0245(3+)

m

AQT(HexNAc)LETPVQAPVLNPEK


Legends

Vitronectin

Q3ZBS7

Thr-142 or Ser-143

v

1054.7689(3+)

m

v

GDSEPGMGTSDLGT(HexNAc)SESPAEEETC(Carbamidomethyl)SGKPF


Legends

Fragments in green indicate that the site of modification may be Thr-142.


Legends

Peptidase inhibitor 16

Q58D34

Thr-408

v

925.4483(2+)

v

SLSNSPSASAT(HexNAc)ANAVGGR


Legends

Fetuin B

Q58D62

Thr-19 & Thr-20

v

914.4683(2+)

v

m

*

T(HexNAc)S(HexNAc)PPQPAARPSSLL


Legends

both ends are non-tryptic

Precursor mass error = -2.3 ppm

Score = 22.1; E = 0.012


Legends

Fetuin B

Q58D62

Thr-157

v

723.9690(3+)

m

TC(Carbamidomethyl)PDC(Carbamidomethyl)PS(HexNAc)TSPYDLSNPR


Legends

Fetuin B

Q58D62

Thr-173

v

723.8355(2+)

v

FM(Oxidation)ETAT(HexNAc)ESLAK


Legends

Fetuin B

Q58D62

Ser-262

v

727.8453(2+)

v

SFFNS(HexNAc)QAPTPR


Legends

Fetuin B

Q58D62

Thr-273

v

595.9606(3+)

v

v

GENAT(HexNAc)VNQRPANPSK


Legends

Fetuin B

Q58D62

(Thr-295) & Thr-299

v

777.0660(3+)

v

m

*

LQQQNTAPT(HexNAc)NSPT(HexNAc)KAVPK


Legends

Pigment epithelium-derived factor

Q95121

Thr-34

v

804.3722(3+)

v

m

AGSLTPEST(HexNAc)GAPVEEEDPFFK


Legends

This UniProt entry (recently deleted) indicated a Leu -> Val substitution at position-403 of kininogen-1, here we show ETD data confirming the mutation

F1MNV4

v

702.8290(2+)

-HexNAc

c11

v

z8*

z10

z9*

c8*

z11

z6*

z7

c6

z5*

*

TEGSTT(HexNAc)VSVPHS


Legends

This UniProt entry (recently deleted) indicated a Leu -> Val substitution at position-403 of kininogen-1, here we show HCD data confirming the mutation

F1MNV4

702.8290(2+)

HexNAc

MH+

y3

b2

b9-2xH2O

y2

y10

y5

y4

TEGSTTVSVPHS + HexNAc

All fragments are within 10 ppm


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