Urinalysis tidbits
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Urinalysis Tidbits. Stains. Sternheimer-Malbin Crystal Violet and Safranin O Lipid Stains Oil Red O and Sudan III Stain Trigs and Neutral Fat but Not Cholesterol Cholesterol polarizes Cholesterol Drops Maltese Cross Cholesterol Crystals Birefringence. Microscopic Sediment Prep.

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Urinalysis Tidbits

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Urinalysis tidbits

Urinalysis Tidbits


Stains

Stains

  • Sternheimer-Malbin

    Crystal Violet and SafraninO

  • Lipid Stains

    • Oil Red O and Sudan III

    • Stain Trigs and Neutral Fat but Not CholesterolCholesterol polarizes

    • Cholesterol Drops Maltese Cross

    • Cholesterol Crystals Birefringence


Microscopic sediment prep

Microscopic Sediment Prep

  • 12 mL

  • Spin 5 min at 400 RCF

  • RCF Relative Centrifugal Force

  • RCF = 1.118 x 10-5 x radius in cm x RPM2

  • Examine a Min of 10 Fields Low and High


Microscopy

Microscopy

  • Bright field

  • Polarized

  • Phase Contrast

  • Interference


Red blood cells

Red Blood Cells

  • Pale red

  • Ghost Cells Dilute or Alkaline Lyse

  • Hypertonic Conditions Crenate

  • 2% Acetic Acid Lyse

  • Yeast and Calcium Oxalate do not disappear with 2 % acidic acid


Urinalysis tidbits

WBC

  • Granular and often visible nucleus

  • Hypertonic will shrink

  • Hypotonic “Glitter” cells associated with Infection and Irritation & Pyelonephritis

  • Glitter cells show Brownian movement


Epithelial cells

Epithelial Cells

  • Squamous Contamination

  • TransitionalBladder

  • Renal Tubular Renal Tubules


Squamous cells

Squamous Cells

  • Large, flat irregular shape

  • Small nucleus

  • Urethra and contamination

  • Not considered Clinically Sig


Clue cell

Clue Cell

  • Vaginal Infection w Gardnerella vaginalis

  • Squamous cell covered w coccobacillus

  • Covers most of cell and extends beyond edges

  • Vaginal wet prep but may appear in urine


Renal tubular cell

Renal Tubular Cell

  • Slightly larger than WBC

  • Eccentric nucleus

  • Increased numbers Tubular damage

  • Pyelonephritis, Acute Tubular necrosis


Hemosiderin

Hemosiderin

  • Not birefringent unlike urates or phosphates

  • Prussian blue dye stains iron of Hb blue


Transitional cells

Transitional Cells

  • Smaller than Squamous

  • Several Forms Caudate, spherical and polyhedral

  • Central nucleus

  • Renal Pelvis, calyses, ureters and bladder

  • Not Clinical Sig catheterization


Starch granules

Starch granules

  • Source Powdered Gloves

  • Maltese cross Polarized light

  • Look like a pouch


Urinalysis tidbits

Crab

  • Pediculosis pubis


Pinworm

Pinworm

  • Enterobiusvermicularis

  • Ova one side flat

  • Adult male


Schistosoma hematobium

Schistosoma hematobium

  • World wide problem Egypt, Iraq, Syria, Iran Africa

  • US lacks the snail intermediate host

  • Trematode Terminal spine


Triple phosphate

Triple Phosphate

  • Tri Phosphate going into solution


Urinalysis tidbits

RTE


Urinalysis tidbits

Mite

  • Adult mite

  • Scabies

  • Smaller than crab


Ua web sites

UA Web Sites

  • Comprehensive Review

  • http://www.aafp.org/afp/2005/0315/p1153.html


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