TELOMERES & TELOMERASE. Prasamit Saurav Baruah MODERATOR Dr. Nalini K. Objectives :. Introduction Overview of DNA replication End replication problem Telomeres – structure & functions Telomerase – hTR & hTERT Telomere replication Regulation of Telomerase TRAP assay _ overview
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Prasamit Saurav Baruah
Dr. Nalini K.
Overview of DNA replication
End replication problem
Telomeres – structure & functions
Telomerase – hTR & hTERT
Regulation of Telomerase
TRAP assay _ overview
Telomeres & cancer
Germ line cell
hTERT transduced cell
Abrogation of p53
– RNA component serving as a template for telomeric synthesis.
- catalytic protein with Reverse Transcriptase activity.
Protein Interacting region Function
TEP1 aa 1–350, 601–927 Unknown
P23/p90 aa 1–195 Assembly/conformation
14-3-3 aa 1004–1132 Nuclear localization
TEP1 nt 1–871 Unknown
hGAR1 hTR H/ACA domain Stability, maturation, localization
Dyskerin/NAP57 hTR H/ACA domain Stability, maturation, localization
hNOP10 hTR H/ACA domain Unknown
hNHP2 hTR H/ACA domain Stability, maturation, localization
C1/C2 nt 33–147 Stability, maturation, localization
La nt 1–205, 250–451 Accessibility to telomeres?
A1/UP1 nt 1–208 Unknown
hStau nt 64–222 Accessibility to telomeres?
L22 nt 64–222 hTR processing, localization?
Telomeres are tucked into double strand repeat forming a loop , which may serve to protect the 3’ overhang.
Displacement of TG rich strand internally stabilized by TRF2.
Rest duplex is bound by TRF1.
GRN163L is a 13-mer oligonucleotide N3’ --> P5’ thio-phosphoramidate (NPS oligonucleotide)
directly bind to the RNA component of telomerase at the active site of the enzyme