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Stem Cell Science

Stem Cell Science. A brief update 9/10/08 Gerry Maxwell. Jaenisch and Young 2008 Cell 132:567-582. Jaenisch and Young 2008 Cell 132:567-582. I. Park and G.Q. Daley 2007 Nature Cell biology 9: 871-873. Jaenisch and Young 2008 Cell 132:567-582. Kinetics of reprogramming.

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Stem Cell Science

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  1. Stem Cell Science A brief update 9/10/08 Gerry Maxwell

  2. Jaenisch and Young 2008 Cell 132:567-582.

  3. Jaenisch and Young 2008 Cell 132:567-582

  4. I. Park and G.Q. Daley 2007 Nature Cell biology 9: 871-873.

  5. Jaenisch and Young 2008 Cell 132:567-582

  6. Kinetics of reprogramming • Reprogramming using virally encoded transcription factors in vitro takes many days and not all markers of pluripotency are activated together • In addition, it appears that stochastic events are involved in reprogramming because clonally derived cells do not reprogram in a synchronous fashion

  7. Creation of Patient Specific Cell Lines • Originally published in Science Express on 31 July 2008 • Science 29 August 2008: • Vol. 321. no. 5893, pp. 1218 - 1221 • DOI: 10.1126/science.1158799 • Induced Pluripotent Stem Cells Generated from Patients with ALS Can Be Differentiated into Motor Neurons • John T. Dimos,1* Kit T. Rodolfa,1,2* Kathy K. Niakan,1 Laurin M. Weisenthal,1 Hiroshi Mitsumoto,3,4 Wendy Chung,4,5 Gist F. Croft,4,6 Genevieve Saphier,1 Rudy Leibel,5 Robin Goland,7 Hynek Wichterle,4,6 Christopher. Henderson,4,6 Kevin E Eggan1

  8. In vivo reprogramming of adult pancreatic exocrine cells tocellsZhou,Brown, Kanareck, Rajagopal, and Melton 2008 Nature 07134

  9. A combination of three transcription factors induces insulin+ cells in adult mouse pancreas in vivo. Q Zhouet al.Nature000, 1-6 (2008) doi:10.1038/nature07314

  10. Induced new b-cells remodel vasculature and ameliorate hyperglycaemia. Q Zhouet al.Nature000, 1-6 (2008) doi:10.1038/nature07314

  11. Characteristics of in vivo reprogramming • Like in vitro the inducing factors are only required transiently • Does not appear to require cell division • Occurs as early as 3 days-faster than in vitro (7-30 days in vitro) • Efficiency is up to 20%- much higher than in vitro • Does not appear to involve dedifferentiation as does in vitro reprogramming

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