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Microbiology Practical I. The following slides were contributed by your fellow BIO 251 students…. Use side “ ” key to view slides…. Lab 1: Eukaryotes and Microscopy. Know the parts of the microscope…. Lab 1: Eukaryotes and Microscopy. Paramecium caudatum. Lab 1: Eukaryotes and Microscopy.

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Microbiology Practical I

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Microbiology Practical I

The following slides were contributed by your fellow BIO 251 students…

Use side “” key to view slides…


Lab 1: Eukaryotes and Microscopy

Know the parts of the microscope…


Lab 1: Eukaryotes and Microscopy

Paramecium caudatum


Lab 1: Eukaryotes and Microscopy

Euglena gracilis


Lab 1: Eukaryotes and Microscopy

Amoeba proteus


Lab 1: Eukaryotes and Microscopy

Fungus – Saccharomyces cerevisiae


Lab 1: Eukaryotes and Microscopy

Cyanobacteria ~ Nostoc


~Bacteria slides~

Micrococcus luteus


~Bacteria Slides~

Bacillus subtilis


~Bacteria Slides~

Bacillus anthracis


~Bacteria Slides~

Rhodospirillum rubrum

Sorry not in focus…


~Bacteria Slides~

See other file for Gram Stain, Endospore, Acidfast, and Capsule Staining…

[ Adobe Acrobat of Gram and Endospore ]

Some Endospore Staining ~


Streak Plate:

Know colony characteristics (color, elevation, margin, etc.)


Spread Plate vs. Streak Plate:


Streak Plate:


Spread Plate:

Mixed – Bacillus subtilis & Serratia marcescens


Practical I Unknown Tests:

~ Simple Stain

~ Gram Stain

~ Endospore Stain

~ Acid-Fast Stain (Demo)

~ Capsule Stain (Demo)

~ Motility (Hanging Drop, Wet Mount, Soft Agar Deep)

~ Obligate Aerobe, Facultative Anaerobe, Aerotolerant Anaerobes, Microaerophiles (Gas Pak, Eugon Agar, Thioglycollate Broth)


Gas Pak Anaerobic vs. Aerobic:


Dilution Problems:


Dilution Problems:


Bacterial Growth Curve:

Make sure you know how to:

Use a spectrophotometer to measure absorbance.

Produce a log graph of the calculations.

Calculate generation time from a growth curve.


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