Testing algorithms used at Bureau of Labs Michigan Department of Community Health. Information on testing algorithms for processing and reporting serological assays on arbovirus panel with specific reference to West Nile Virus testing. Slide 1. CSF at MDCH 0 day. Notification of
Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.
Testing algorithms used at Bureau of Labs Michigan Department of Community Health
Information on testing algorithms for processing and reporting serological assays on arbovirus panel with specific reference to West Nile Virus testing
CSF at MDCH 0 day
Via EPIC to
LDH & EPI
IgM ELISA 1stRun
To report a Probable case
request a serum sample
IgM ELISA 2ndRun
Request a convalescent serum sample
Acute Serum at MDCH
PRNT (IgG) on pair
4x rise in titer
Confirmed positive reports
Hold and request Convalescent serum
CNS symptoms +
Testing Approved by Lab Manager
IgM ELISA 1st Run
IgM ELISA 2nd Run
PRNT on acute serum
4.The submitter, Epidemiology and the local health department will be notified of the results via EPIC.
Single serum with Documented CNS symptoms
or paired sera without 4 x increase in titer
IgM ELISA (+) Netralizing Antibody (-)
Test for EEE, SLE and CGV
Single serum collected too early
(0-8 d) after onset of symptoms
No WNV Case
Probable WNV Case
In a few cases there will be results which are more difficult to interpret.
as Not a Case.