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Chapter 10. Homologous Recombination. 27 and 29 September, 2004. Overview. Homologous recombination relies on regions of nearly identical sequence. The Holliday model of the mechanism of recombination involves recognition, strand invasion, branch migration, and resolution.

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Chapter 10

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Chapter 10

Homologous Recombination

27 and 29 September, 2004


Overview

  • Homologous recombination relies on regions of nearly identical sequence.

  • The Holliday model of the mechanism of recombination involves recognition, strand invasion, branch migration, and resolution.

  • The Double-strand Break Repair model is similar, but involves two Holliday junctions.

  • Homologous recombination is mediated by protein enzymes.

  • RecA (Rad51p) pairs homologous regions and promotes strand exchange.

  • RecBCD (Rad 50/58/60p; MRXp) generates single strand for invasion.

  • RuvAB and RuvC (?) promote branch migration and resolution.

  • Homologous recombination is required for successful meiosis.

  • In meiosis, Spo11 generates DSB, MRXp extends the break, and Rad51 promotes strand invasion.

  • Some events, including mating-type switching in yeast and VDJ recombination involve regulated, specific recombination.


Holliday Junction Cleavage and Strand Invasion


Holliday Resolution


DSB Recombination


DNA Damage leads to DSB


RecBCD and Chi Sites


Chi Site Polarity


RecA-catalyzed Strand Exchange


RecA - DNA Structures


Polarity of Cooperative RecA Binding


Mechanism of RecA Strand Exchange


RuvAB and Branch Migration


RuvC Resolves Holliday Structures


Meiotic Recombination


Spo11p forms Double-stranded Breaks


Recombination Mechanism


Mating-Type Conversion


The mechanism of mating-type conversion involves replication to bring about gene conversion.


Genetic and Physical Maps


Gene Conversion by Mismatch Repair


Resolving Paired Holliday Structures


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