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EPSPS and Crops Developed by Dr. M. Zapiola for CSS/Hort 431

EPSPS and Crops Developed by Dr. M. Zapiola for CSS/Hort 431. glyphosate. Glyphosate Herbicide. Glyphosate inhibits EPSPS enzyme - impeding the synthesis of aromatic amino acids. CO 2 H 2 O NH 3. aromatic amino acids. EPSPS. Proteins. CO 2 H 2 O NH 3. aromatic amino acids. EPSPS.

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EPSPS and Crops Developed by Dr. M. Zapiola for CSS/Hort 431

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  1. EPSPS and Crops Developed by Dr. M. Zapiola for CSS/Hort 431

  2. glyphosate Glyphosate Herbicide • Glyphosate inhibits EPSPS enzyme - impeding the synthesis of aromatic amino acids CO2 H2O NH3 aromatic amino acids EPSPS Proteins CO2 H2O NH3 aromatic amino acids EPSPS Proteins

  3. Commercial RR crops "Two key elements needed for the development of commercially viable glyphosate-tolerant crops are: • a resistant target enzyme • sufficient expression of that enzyme within the transgenic plant” Heck et al. 2005. Development and Characterization of a CP4 EPSPS-Based, Glyphosate-Tolerant Corn Event: Crop Sci. 45:329-339 (2005).

  4. glyphosate Roundup Ready Crops • When incorporated into the genome of RR-susceptible plants, the Roundup ReadyTM gene (CP4 EPSPS) confers resistance to glyphosate CO2 H2O NH3 aromatic amino acids EPSPS Proteins CP4 EPSPS

  5. promoter (leading sequence) resistant EPSPS terminator Gene Construct • The gene is introduced into the plant nuclear genome via different mechanisms • The trait is then incorporated to elite varieties by backcrossing

  6. Gene Construct: RR soybean • LOCUS AB209952.1 • Size 2457 bp • SOURCE Glycine max • FEATURES • CaMV35S • Chloroplast transit peptide • CP4EPSPS • Nos 3’ terminator • Repeated fragment of cp4epsps truncate Gene bank gi|62318478|dbj|AB209952.1|[62318478]

  7. PCR Primer Design

  8. Scanning 96 Plants

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