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GMS provides “One stop solution”

GMS provides “One stop solution”. Sacace Biotechnologies Srl “Complete qPCR Solutions”. Founded in 2001. Sacace Biotechnologies s.r.l . , is an innovative Italian company, located in Como, Lombardia. 2001-2009: Caserta, Campania region. Now: Como, Lombardia region. Our Mission.

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GMS provides “One stop solution”

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  1. GMS provides “One stop solution” Sacace Biotechnologies Srl “Complete qPCR Solutions”

  2. Founded in 2001 Sacace Biotechnologies s.r.l., is an innovative Italian company, located in Como, Lombardia 2001-2009: Caserta, Campania region Now: Como, Lombardia region

  3. Our Mission To supply the best products to the international diagnostics and pharmaceutical industry, as well as to hospitals and laboratoriesthrough a global network of distributors located in more than 60 countries all over the world.

  4. Sacace Worldwide

  5. Functions • Research & Development • Manufacturing • Logistics • Marketing • Certifications (ISO 9001:2008 13485:2012, CE Mark - Directive 98/79 EC) • International external quality control

  6. Our PCR Kits • Hepatitis Viruses (Quantitative tests for HCV, HBV, HDV, HGV, HAV detection) • Human Papilloma Viruses (Screening, Typing, Quantitative detection of Low & High Risk genotypes) • STD (Multiplex detection of Chlamydia, Neisseria, Trichomonas, Mycoplasma, Treponema, Candida ….) • Herpes Viruses (Quantitative Multiplex detection of CMV, EBV, HHV6, HSV, VZV)

  7. Our PCR Kits • TORCH infections (Toxoplasma, Rubella, Parvovirus) • Respiratory infections (Tuberculosis, Influenza, hRSV, Bordetella, Pseudomonas, Legionella) • Neurological infections (Enteroviruses, N.meningitidis, H.influenza, Str.pneumoniae) • Gastrointestinal infections (Shigella, Salmonella, E.coli, Rotavirus, Astrovirus, Norovirus, H.pylori)

  8. Our PCR Kits • Single Nucleotide Polymorphisms (SNP): • Cardio Trombophilia Panel (FII, FV, FVII, FXIII, FGB) • Cardio Hypertension Panel (ADD, AGT, NOS....) • Folates Methabolism Panel (MTHFR, MTR, MTRR) • Haemochromatosis Panel (HFE 187, HFE193, HFE845) • Warfarin Panel (CYP2C9, CYP4F2, VKORC1)

  9. Our PCR Kits • Oncologic diseases (Mbcr-ABL, BRCA) • Genetically Modified Organisms (Soya, Corn, Rice, Tomatoes) • Veterinary kits (CPV, PPV, CSVF, BVDV, ASF, Brucella, TGEV...) • RNA/DNA extraction kits

  10. Our PCR Kits Our Real Time PCR kits can work on multiple instruments: • Qiagen Rotorgene TM • Bio-Rad iQ5TM • Cepheid SmartCycler TM • Applied Biosystems 7300/7500/StepOne TM • Stratagene MX3005P TM • Roche LightCycler 2.0 TM • Bioer LineGene-K TM • SaCycler-96 TM from Sacace Biotechnologies • ...more to come

  11. SaCycler™-96

  12. SaCycler™-96 Features • 4 or 5 channels multiplexing for discrimination of up to five targets in a single reaction well • Strong flexibility thanks to the 96-well format suited for standard PCR microplates, test tubes and strips • Possibility to use tubes with different heights with regulation of the matrix block • Optimal signal/noise ratio and absence of crosstalk ensured by the unique design of the optical track including a separate light source for each channel and a matrix CCD camera • Light emitting diodes (LED) as a light source with a lifetime of about 100,000 hours that does not require maintenance

  13. SaCycler™-96 Features • Wide dynamic range of detection using multiple exposure method, which leads the optimization of signal registration conditions to a whole new level, greatly simplifying or even eliminating the need for fluorescence settings • Main applications are Real-Time quantitation, single nucleotide polymorphisms (SNPs) genotyping, melting curve and gene expression analysis • Instrument comes with the most used Sacace protocols already installed in the software minimizing possibility of error during programming.

  14. SaCycler™-96 Characteristics • Thermal block format 96 test tubes of 0.2 ml (12 х 8) • Test tube type 0.2-ml test tubes for PCR (individual, in strips, 8 pieces each or a holder 12 x 8) • Range of thermal block temperature control 0 C…100°С • Resolution of temperature setting 0.1°С • Absolute accuracy of temperature maintenance±0.2°С • Uniformity of thermal block temperature ±0.15 °С • Maximum heating rate of the thermal block within temperature range of 4…99 °С3.5 °С /s • Maximum cooling rate of the thermal block within temperature range of 99….55 °С 2.5°С /s • “Hot cover” temperature105°С1°С • Actuating device of the thermal block Peltier elements • Excitation sourceLight-emitting diode (LED) • DetectorCCD (charge coupled device) -matrix • Number of the fluorescence measurement channels4 or 5 • Excitation/detection wave length 470/525, 532/570, 585/633, 633/670, 690/750

  15. SaCycler™-96 Comparison

  16. Sacacethermalcycling protocols 38 different kits

  17. Sacacethermalcycling protocols 2 different kits 4 different kits 2 different kits 7 different kits

  18. Thanks for your attention

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