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Homeostasis: Osmoregulation in elasmobranchs







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Homeostasis: Osmoregulation in elasmobranchs. The difference between marine, eurahyline and fresh water species. Osmoregulation. Relationship between solute to solvent concentrations of internal body fluids The environment the organism lives in Isotonic? Hypertonic? Hypotonic?.
Homeostasis: Osmoregulation in elasmobranchs

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Slide 1

Homeostasis: Osmoregulation in elasmobranchs

The difference between marine, eurahyline and fresh water species

Slide 2

Osmoregulation

  • Relationship between solute to solvent concentrations of internal body fluids

  • The environment the organism lives in

  • Isotonic?

  • Hypertonic?

  • Hypotonic?

Osmolarity = solute/solvent concentration

Slide 3

water molecules

protein molecules

semipermeable membrane

between two compartments

Fig. 5-20, p.86

Slide 4

2% sucrose solution

1 liter of

10% sucrose solution

1 liter of

2% sucrose solution

1 liter of distilled water

Hypotonic

Conditions

Hypertonic

Conditions

Isotonic

Conditions

Fig. 5-21, p.87

Slide 5

first compartment

second compartment

hypotonic

solution

hypertonic

solution

membrane permeable

to water but not to solutions

fluid volume

rises in second

compartment

Fig. 5-22, p.87

Slide 6

Stepped Art

Hypotonic

Solution

Hypertonic

Solution

membrane permeable to

water but not to solutes

Fig. 5-22, p.87

Slide 7

The Challenge

  • Avoid desiccation in an aqueous environment

    • MARINE ANIMALS

      • Dehydration

      • Elimination of excess salt

  • FRESHWATER ANIMALS

    • Conserve salts

    • Eliminate excess water

Slide 8

Environmental challenges of elasmobranchs

  • All ureotelic and ureosmotic except potamytrygonid rays

  • Marine elasmobranchs surrounded by salt; lose water;

    • need to get rid of excess organic and inorganic compounds

  • Euryhaline species environment fluctuates

    • Must handle salt and fresh conditions

  • Freshwater species

    • lose salt and electrolytes; need to get rid of excess water

Slide 9

Dealing with Environment

  • Marine : Maintain serum osmolarity = or greater than seawater primarily w/ urea

    • Little osmotic loss of water

  • Dilute Seawater or Freshwater: Serum osmolarity reduced

    • Little diffusion of water inward

Slide 10

Players in osmoregulation

  • Organs

    • Kidney, liver, gills, rectal gland

  • Organic compounds

    • Urea

    • TMAO trimethylamine oxide

  • Inorganic ions

    • Sodium

    • Chloride

    • Other salts

Slide 11

Umanitoba - Gary Anderson

Slide 12

Body FluidMarine Elasmobranchs

  • Reabsorb & retain urea and other body fluid solutes in tissues

  • Serum osmolarity remains just greater than external seawater (hyperosmotic)

  • Don’t have to drink water like teleosts

  • Water gained excreted by kidneys

  • Tri-MethylAmine Oxide (TMAO): Acts to counteract the perturbing effects of urea

Slide 13

Marine elasmobranchsPlasma solutes and osmoregulation

  • Different than marine teleosts

  • Have high osmolarity

  • Reabsorb and retain high levels of urea and TMAO in their body fluids

  • Osmolarity remains hyperosmotic to surrounding seawater

  • TMAO to stabilize proteins and activate enzymes

  • Water gained across gills is excreted by kidneys

  • Any salt gained across gills is excreted by rectal gland and kidney

Slide 14

Body fluid of euryhaline elasmobranchs

  • Ammonotelic in frehwater

  • As salinity increases

    • Increase urea production and retention

    • Decrease urea excretion

    • Increase Na+ and Cl-

    • Decrease ammonia excretion

  • Can not produce and retain as much urea as marine spp. (lower osmolarity)

  • Ex. D. sabina and H. signifier

Slide 15

Body fluid of euryhaline elasmobranchs

As salinity decreases

  • Lower osmolarity (less urea and TMAO) than marine species

  • Decrease amount of urea produced and reabsorbed

  • Increased urinary excretion

  • Loss of sodium and chloride balanced by electrolyte uptake at the gills and reabsorbed by kidneys

Slide 16

Bull Shark - Carcharhinus leucas

Eeigen Werk

Slide 17

Body FluidFresh Water Elasmobranchs

  • Lost ability to synthesize and retain urea or TMAO

  • Body fluid solute concentrations relatively low

  • Freshwater rays abandoned renal reabsorption

    • Urine is dilute

    • Ammonotelic

  • Ex. Potamotrygon rays

Slide 18

Potamotrygonidae

Raimond Spekking

Slide 19

Urea- production, retention and reabsorption

  • Urea production

    • Occurs in the liver

  • Retention

    • In gills

  • Reabsorption

    • In kidneys

Slide 20

Urea production in liver

Ornithine-urea cycle (OUC)

  • Glutamine synthetase is crucial enzyme needed for urea production

  • Euryhaline spp. decrease production of urea when entering fresh water

  • Freshwater rays lack the enzyme for the biosynthesis to occur

  • Unsure if urea is produced in other locations

  • Bacteria hypothesized for being responsible

Slide 21

Marine gills retain urea

  • Do not lose much urea across gills

  • Gill’s basolateral membrane has high cholesterol to phospholipid ratio levels

    • Membrane limit diffusion

  • Active transport of urea by Na+/ urea antiporter energized by Na+/K+ ATPases

  • Used more for salt regulation and acid/base balance

Slide 22

Kidneys reabsorb urea

  • Reabsorption contributes to high urea levels

  • Minor site of urea loss

  • Thought to involve active transport

  • Use urea-sodium pump

  • Proven in R. erinacea

  • Second hypothesis for passive transport that has not been proven

  • Euryhaline spp. decrease renal reabsorption of urea as enter areas of decreased salinity

    • Increases rate of urine flow to rid system of excess urea

Slide 23

Salt regulation

  • Rectal gland secretions

    • Marine spp. surrounded by high salinity

    • Rectal gland secretes sodium and chloride

    • Na+/ K+ ATPases used

Slide 24

Osmoregulation by the Rectal Gland

  • Rectal Gland = Salt secreting mechanism

    • Migratory elasmos - regressive rectal gland

    • Non-functional in freshwater rays

http://fig.cox.miami.edu/~cmallery/150/physiol/rectal.htm

Slide 25

Salt regulation

  • Gills

    • Salt uptake

      • Na+/ K+ ATPases even higher in freshwater

    • Acid/ base balance

      • Secrete acid

      • H+ excreted/exchanged for Na+

      • Run by Na+/ K+ ATPases

    • Responsible for ammmonia secretion

Slide 26

Salt regulation

  • Kidney salt excretion

    • Dilute environment

      • Urine flow increase

    • Saltwater

      • Not solely responsible for salt secretion

Slide 27

Endocrine Regulation to Regulate Body Fluid Volume and Solute Concentration

  • CNP - Released from heart

    • Increase urine production

    • Stimulate salt secretion from rectal gland

    • Inhibit drinking and relax blood vessels

  • AVT

    • Increase in plasma osmolality

    • Reduces urine production

  • RAS

    • Antagonistic to CNP, reduces urine flow

    • Increases drinking

    • Constricts blood vessels

Slide 28

Feeding and osmoregulation

  • Urea is metabolically expensive

    • 5 umol ATP for 1 mole urea

  • Protein in food is main source of N in urea

  • Elasmobranches must get adequate food to produce the urea

  • Why ureotelic and not ammonotelic???

Slide 29

Literture cited

  • Hammerschlag N.2006. Osmoregulation in elasmobranches: a review for fish biologists, behaviorists and ecologists.MARINE AND FRESHWATER BEHAVIOUR AND PHYSIOLOGY 39 (3): 209-228

  • Speers-Roesch B, Ip YK, Ballantyne JS.2006. Metabolic organization of freshwater, euryhaline, and marine elasmobraches: implications for the evolution of energy metabolism insharks andrays. JOURNAL OF EXPERIMENTAL BIOLOGY 209 (13): 2495- 2508

  • Pillans RD, Anderson WG, Good JP, et al.2006. Plasma and erythrocyte solute properties of juvenile bull sharks, Carcharhinus leucas, acutely exposed to increasing environmental salinity. JOURNAL OF EXPERIMENTAL MARINE BIOLOGY AND ECOLOGY 331 (2): 145-157

  • Pillans RD, Good JP, Anderson WG, et al. 2005.Freshwater to seawater acclimation of juvenile bull sharks (Carcharhinus leucas): plasma osmolytes and Na+/K+ ATPase activity in gill, rectal gland, kidney and intestine. JOURNAL OF COMPARATIVE PHYSIOLOGY B-BIOCHEMICAL SYSTEMIC AND ENVIRONMENTAL PHYSIOLOGY 175 (1): 37-44

Slide 30

Literature cited

  • Pillans RD, Franklin CE.2004. Plasma osmolyte concentrations and rectal gland mass of bull sharks Carcharhinus leucas, captured along a salinity gradient. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A- MOLECULAR & INTEGRATIVE PHYSIOLOGY 138 (3): 363-


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