1 / 16

Malaria Lab Session

Malaria Lab Session. COL Art Lyons , PhD,MD February 26, 2014 Fort Riley Trop. Med. Course. Helpful Reference:. WHO Health Library for Disasters http://helid.digicollection.org/en Search for ‘malaria’ Basic Malaria Microscopy – Learner’s Guide

trevor
Download Presentation

Malaria Lab Session

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Malaria Lab Session COL Art Lyons, PhD,MD February 26, 2014 Fort RileyTrop. Med. Course

  2. Helpful Reference: • WHO Health Library for Disasters • http://helid.digicollection.org/en • Search for ‘malaria’ • Basic Malaria Microscopy – Learner’s Guide • http://whqlibdoc.who.int/publications/2010/9789241547826_eng.pdf

  3. Blood Smear for malaria Thick Film Thin Film Red blood cells layered 10-20 times thicker v. thin film Red Cells are not fixed (staining caused lysis) thus the parasites are within red cell ‘ghosts’ Can evaluate large amount of red blood cells Sensitive (negative more likely to rule out malaria) Single layer of red blood cells Fixed with methanol Used to identify parasite species. Used to estimate parasitemia View after thick smear Specific STAINING: http://www.dpd.cdc.gov/dpdx/HTML/PDF_Files/malaria_staining_benchaid.pdf

  4. Chromatic = redCytoplasm = blueDon’t see both = Not malaria

  5. Key Morphological Differences Between Human Plasmodium Species in Blood Smears

  6. P. falciparum

  7. P. vivax Giemsa – Schuffner’s dots

  8. P. ovale Coarse Pigment Compact troph.

  9. P. malariae Unlike Vivax, Not Enlarged

  10. The End

More Related