FLUORIMETRY AND PHOSPHORIMETRY. INTRODUCTION. Fluorescence is the emission of visible light by a substance that has absorbed light of a different wavelength. The emitted photon has a longer wavelength.
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FLUORIMETRY AND PHOSPHORIMETRY
• Nonradiative deactivation
• Radiative deactivation
The common factors affecting the fluorescence are as follows.
• Dissolved oxygen
• Excitation light sources
•Filters or Monochromators
• Sample holder
• Readout device
1) A Rotating-shutter device commonly called a phosphoroscope and
2) a sample system which is maintained at liquid nitrogen temperature.
2)The Becquerel or rotating disc phosphoroscope: