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Molecular Biology

Spring 2009 BI 425 UOG. Molecular Biology. Topics for Second Part of Molecular Biology Course: Structural Organization of Eukaryotic Chromosomes. NonTranscribed genes are less susceptible to Dnase 1 Digestion. Fluorescent Labeled Probes hybridized to Interphase Chromosomes.

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Molecular Biology

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  1. Spring 2009 BI 425 UOG Molecular Biology Topics for Second Part of Molecular Biology Course: Structural Organization of Eukaryotic Chromosomes

  2. NonTranscribed genes are less susceptible to Dnase 1 Digestion

  3. Fluorescent Labeled Probes hybridized to Interphase Chromosomes

  4. Prokaryotic Gene Regulation

  5. Deletion Analysis: Identify Upstream Transcription Control Sequences

  6. Determination of Consensus TATA Box Sequence

  7. Scanning Mutations Identify Transcription Control Elements

  8. General Pattern of Control Elements that Regulate Gene Expression in Yeast and other Eukaryotes.

  9. LIFE CYCLE OF HIV-1 HIV Virus Latency DNA Provirus RNA Viral DNA Transcription Viral RNA RNA Processing Viral genomic RNA mRNA Virion Assembly Viral Proteins Maturation & Release of Virus if rev 3’ LTR vpu vpr pol env nef Shubir Ghosh, UCSF

  10. Shubir Ghosh, UCSF

  11. Overview of Eukaryotic Transcriptional Control

  12. Column Chromatography Separation of the three RNA Polymerases In Eukaryotes.

  13. Transcription Assay: Transcription Start Site

  14. EMSA

  15. Dnase 1 Footprinting

  16. Dnase1 Footprint Assay in Transcription Factor Purification

  17. Northern Blot & S1 Nuclease Assay: Identify Enhancers of Transcription Northern Blot S1 Nuclease Assay Homework!

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