Prostate transverse section
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Prostate: Transverse section. Radical Prostatectomy. Bladder neck. Apex. Radical Prostatectomy. Base/ Bladder Neck. Apex. Posterior quadrants of alternate slices have been removed and frozen after inking the surface. Lateral aspect.

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Prostate: Transverse section

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Prostate transverse section

Prostate: Transverse section


Radical prostatectomy

Radical Prostatectomy

Bladder neck

Apex


Radical prostatectomy1

Radical Prostatectomy

Base/

Bladder Neck

Apex

Posterior quadrants of alternate slices have been removed and frozen after inking the surface


Prostate transverse section

Lateral aspect

Schematic of how samples of tumor are obtained from a radical prostatectomy specimen, without compromising sampling or diagnostic pathology data

Seminal

Vesicle

Permanent section

Permanent section

right

left

1

7

2

6

3

5

4

5R

5

5R5L

5L

Fresh, non-frozen tissue

for flow sorting

Frozen section

Unfixed tissue

for RNA & DNA

left

right

4R

Frozen section

4

4R4L

4L

Permanent section

left

right

permanent section

3R

3

3R5L

3L

= Prostate carcinoma


Prostate transverse section

Human prostate (lateral view)

Obtaining samples of non-tumortissue from a radical prostatectomy specimen.

Seminal

Vesicle

Permanent

section

Fixed tissue:

In-situ hybridization

right

1

left

7

2

6

3

5

4

5R

5

5R5L

5L

Fresh, non-frozen tissue: cell sorting

This block is snap frozen (isopentane in liquid N2), within < 20 minutes of devitalization

Frozen tissue:

Immunostains

Laser capture

right

left

4R

4

4R4L

4L

Frozen section

Permanent

section

left

right

Permanent

section

3R

3

3R5L

3L

= Prostate carcinoma


Prostate transverse section

Prostate (transverse section)


Normal prostate parenchyma high magnification

Normal prostate parenchyma (high magnification)

Basal epithelial cells

Secretory (luminal) epithelial cells

Fibromuscular stromal cells


Normal prostate parenchyma low magnification

Normal prostate parenchyma (low magnification)

fibromuscular stroma

normal glands

lymphocytes


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