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CSF: Current DIAGNOSTIC in Cuba. Boca Chica, RD. Marzo/2004. Familia Flaviviridae. Género Pestivirus. PPC. BD. BVD. Host. Proteins. HOMOLOGY: 85 %. Genome. HOMOLOGY: 66 - 74. %. Antibodies. CROSS SEROLOGY. ¿ What is internationally done?.

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CSF: Current DIAGNOSTIC in Cuba

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CSF: Current DIAGNOSTIC in Cuba

Boca Chica, RD. Marzo/2004


Familia

Flaviviridae

Género

Pestivirus

PPC

BD

BVD

Host

Proteins

HOMOLOGY: 85 %

Genome

HOMOLOGY: 66 - 74

%

Antibodies

CROSS SEROLOGY


¿What is internationally done?

  • EUROPE: ELISA Ab, ELISA Ag, DIF, Virus Isolation, PCR, sequencing (Germany)

  • AMERICA: DIF, ELISA Ab, sequencing (Cuba, USA, Mexico)

  • ASIA: DIF, NPLA, PCR, ELISA Ag, sequencing (Korean Republic).


Clínico-Epizootiológico

DIAGNÓSTICO

VIROLÓGICO

Anatomopatológico

Bacteriológico

National Program for CSF


DIAGNOSTIC PROCEDURESIdentification of the virus and/or its components


DIAGNOSTIC PROCEDURESAntibodies identification


  • CURRENT LABORATORY DIAGNOSTIC IN CUBA :

  • Samples (T,S,LN) from animals with more than 30 days of vaccination.

  • Freezing section of tonsils, spleen, lymphatic nodes

  • DIF with national polyclonal conjugate

    DIAGNOSTIC REPORT …. In 72 hours

  • Maceration of organs pool (T,S and LN)

  • Inoculation in PK-15 or SK-6 cells/Virus isolation

  • Virus identification: DIF or DIP/TC, RT-PCR supernatant/diagnostic or sequencing

  • Genotyping

    DIAGNOSTIC REPORT ….. In 30 days


Samples processed in 2003 by DIF

Per productive sectors

TOTAL= 221/196


Samples processed in 2003 for viral isolation

Per productive sectors

TOTAL= 16/9+


  • Composed by :

  • Head of the CSF Program (IMV)

  • Specialists from IMV (Surveillance)

  • Specialists from CENEDI (Pathology, Epidemiology)

  • Specialists from CENSA (Virology)

  • IMV Specialists in swine (Epidemiology)

  • Havana provinces

  • Veterinary Chief from GRUPOR,

  • From Genetic or other main producers as it is the case

National Comission for the diagnostic of CSF

Analysis of the lab results for FOCI declaration (actually ….. 15 days)


DATA TREATMENT

All the outcomes are the objective to be transmit to the “Sistema de vigilancia epidemiológica molecular de la PPC” for further analysis


QUALITY ASSURANCE

  • Internal Validation of reactive

  • PNO

  • RPNO

  • Internal Auditoria

  • External Auditoria

  • GLP


Mutation rates


CURRENT CRITICAL POINTS IN DIAGNOSTIC

Differential diagnostic from other related diseases

Differential diagnostic from the vaccinal virus

MAbs Cj for routine diagnostic

ELISA’s commercially

To continue the genotyping of isolates

Diagnostic procedures for carriers

Cryostat


Immediate Outlooks

  • Training of specialist from CENEDI at CENSA to assume the primary diagnostic by DIF in freezing sections

  • Accreditation of the virology lab at CENSA as a Regional Reference Laboratory

  • To assign CENEDI the necessary resources for the diagnosis


For a Caribbean CSF free in 2008

M A N Y T H A N K S....!!


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