1 / 13

Warm-up

Warm-up. List 3 new things you learned about bacteria. Genetic engineering. Recombinant DNA technology. Why are we using bacteria for biotechnology rather than some other organsism?. What are some characteristics that make bacteria a good choice for genetic engineering?

titus
Download Presentation

Warm-up

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Warm-up • List 3 new things you learned about bacteria.

  2. Genetic engineering Recombinant DNA technology

  3. Why are we using bacteria for biotechnology rather than some other organsism? • What are some characteristics that make bacteria a good choice for genetic engineering? (think and then talk with your partner)

  4. Bacterial plasmids Most bacterial DNA is on a single large chromosome, but some DNA is in small circles called plasmids. • Plasmids can have genes which make bacteria resistant to antibiotics • Plasmids can be passed between bacteria

  5. Plasmids can be manipulated easily in the laboratory • Plasmids can be collected from bacteria • Restriction enzymes can cut specific sequences of DNA • Ligase (enzyme) can paste pieces of DNA together • Bacteria can be transformed by taking in plasmids given to them

  6. Cutting and Pasting DNA • Restriction enzymes act like molecular scissors – they cut the DNA! • Ligaseacts like molecular glue – it is used to reconnect the DNA that has been cut by restriction enzymes.

  7. GATCC G G CCTAG A Restriction Enzyme: BamH I

  8. Transforming Bacteria • When a bacterial cell takes in a plasmid from the environment, it has new DNA (and therefore, new traits). • Scientists say that the bacteria has been transformed.

  9. Exit PowerPoint and open files in Internet Explorer: • Gene engineering cartoon.swf • Gene engineering video.swf

  10. How to produce human insulin from bacteria • Isolate plasmid DNA from bacteria and insulin gene from human. • Cut both DNAs with the same restriction enzyme. • Mix the DNA together with ligase. • Insert the new DNA into bacteria (transformation). • Use antibiotics to kill any bacteria without the plasmid. • Grow bacteria and harvest the insulin.

  11. How to produce human insulin from bacteria • Isolate plasmid DNA from bacteria and insulin gene from human. • Cut both DNAs with the same restriction enzyme. • Mix the DNA together with ligase. • Insert the new DNA into bacteria (transformation). • Use antibiotics to kill any bacteria without the plasmid. • Grow bacteria and harvest the insulin.

  12. Read in book (Owl) p. 239-242. • Draw a diagram showing the six steps to introduce a foreign gene into bacteria.

More Related