Yeast Vector System. Laboratory of Molecular & Cellular Proteomics Park Youn Jin. Introduction. yeast episome plasmid, YEp yeast integrative plasmids, YIp yeast centromere plasmid, YCp . YIp Vectors . - PowerPoint PPT Presentation
Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.
Yeast Vector System
Laboratory of Molecular & Cellular Proteomics
Park Youn Jin
About 400 origins exist in the 17 chromosomes of S. cerevisiae Each yeast origin sequence, called an autonomously replicating sequence (ARS), confers on a plasmid the ability to replicate in yeast and is a required element in yeast artificial chromosomes Only one essential element is 15-bp segment designated element A. Three other short segments-the B1, B2 and B3 elements-are required for efficient functioning of ARS1 A complex of proteins called ORC(origin-recognition complex) binds specifically to element A in ARS1 in an ATP-dependent manner