Yeast Vector System. Laboratory of Molecular & Cellular Proteomics Park Youn Jin. Introduction. yeast episome plasmid, YEp yeast integrative plasmids, YIp yeast centromere plasmid, YCp . YIp Vectors .
Laboratory of Molecular & Cellular Proteomics
Park Youn Jin
About 400 origins exist in the 17 chromosomes of S. cerevisiae Each yeast origin sequence, called an autonomously replicating sequence (ARS), confers on a plasmid the ability to replicate in yeast and is a required element in yeast artificial chromosomes Only one essential element is 15-bp segment designated element A. Three other short segments-the B1, B2 and B3 elements-are required for efficient functioning of ARS1 A complex of proteins called ORC(origin-recognition complex) binds specifically to element A in ARS1 in an ATP-dependent manner