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Chapter 11. Transposition and Site-Specific Recombination. 29 September and 1 October, 2004. Overview. Conservative Site-Specific Recombination (CSSR) may involve insertion, deletion, or inversion of DNA sequences.

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Chapter 11

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Chapter 11

Transposition and Site-Specific Recombination

29 September and 1 October, 2004


Overview

  • Conservative Site-Specific Recombination (CSSR) may involve insertion, deletion, or inversion of DNA sequences.

  • Site-specific recombinases have a mechanism that includes a DNA-protein covalent intermediate.

  • CSSR may be regulated by the presence or absence of accessory proteins.

  • Resolvases are CSSR recombinases that disentangle circular chromosomes.

  • Transposons move using recombination pathways.

  • Transposons may be autonomous or nonautonomous.

  • DNA transposons and viral-like retrotransposons move via a cut/paste mechanism.

  • Retrotransposon movement involves reverse transcription.

  • Some transposons regulate copy number or control target site selection through the use of proteins or antisense RNA.

  • V(D)J recombination uses regulated, specific recombination to generate immune diversity.


Recombination and Transposition


CSSR: Prophage Insertion


Three Classes of CSSR


Recombinase Recognition Sites


Recombinase Mechanism


Serine Recombinases


Tyrosine Recombinases


Mechanism of Cre Recombinase


Cre-DNA Structure


Lambda integration requires architectural proteins.


lInt and IHF stabilize bent DNA.


Hin Inversion


Hin inversion requires Fis bound at an enhancer.


Resolvases disentangle circular DNAs after replication.


FtsK Regulation of the Xer Resolvase Mechanism


FtsK is present at the division closure site.


Conservative and Replicative Transposition


Transposons in Several Genomes


Types of Transposons


Cut and Paste Transposition


Three Mechanisms for Cleaving the Nontransferred Strand


Replicative Transposition


Retrotransposon Movement


Retrotransposon Movement


Retrotransposon Movement


DNA Transposases and retroviral integrases are members of the same protein superfamily.


LINE poly-A Retrotransposon Movement


Tn10 achieves antisense copy control by overlapping promoters.


Antisense Copy Control


Tn10 transposase promoter is active only when hemimethylated.


MuA and MuB participate in selecting Mu transposition target sequences.


MuA disrupts MuB assembly, conferring transposition target immunity.


Clustered Integration of Yeast Ty Elements


Antibody


V(D)J Recombination


Recombination Signal Sequences


V(D)J Recombination Mechanism


Title


LINES and SINES Again?


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