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Isolation, maintenance and screening of symbiotic and non symbiotic diazotrophs BY Ariba Ajmal 2010-ag-3582 From Soil Bacteriology Section Ayub Agriculture Research institute (AARI), Faisalabad Supervised By Muhammad Amjad Qurashi. Research work.

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Isolation, maintenance and screening of symbiotic and non symbiotic diazotrophs

BY

AribaAjmal

2010-ag-3582

From

Soil Bacteriology Section

Ayub Agriculture Research institute (AARI), Faisalabad

Supervised By

Muhammad AmjadQurashi


Research work
Research work symbiotic

  • Case study; Growth Promotion Potential of Different PGPR on the Growth of Maize

  • Media preparation and isolations

  • Soil analysis


Case study growth promotion potential of different pgpr on the growth of maize
CASE STUDY symbiotic GROWTH PROMOTION POTENTIAL OF DIFFERENT PGPR ON THE GROWTH OF MAIZE.


Introduction
INTRODUCTION symbiotic

  • Maize (Zeamays L.).

    • Most diffused crop world wide

    • 3rd most important crop after wheat and rice.

    • Use as both grain and fodder.

    • High economic and nutritional value

    • High starch content about 75% of weight

    • Maize kernels provide significant amount of proteins, involved in N storage


PGPR symbiotic

  • Plant growth promoting rhizobacteria

  • potential tools for sustainable agriculture

  • Rhizobacteria are rhizosphere competent bacteria that aggressively colonize plant roots; they are able to multiply and colonize all the ecological niches.

  • Alternative source of mineral fertilizers


Objectives
Objectives symbiotic

Keeping in view the above discussion, the present study

was designed with the following objectives:

  • Isolation and screening of effective rhizobia and PGPR

  • Testing potential of selected rhizobia and PGPR strains alone as well as in combination (co-inoculation) for improving growth, yield and nodulation of maize.

  • Identification and characterization of selected rhizobia and PGPR that are beneficial for improving growth of maize.  


Rhizobium chickpea
Rhizobium symbiotic Chickpea

  • One of the most important dry land field and pulse crops.

  • Mainly grown in rainfed areas

  • Leguminous crop

  • Role play in BNF

  • Maximum N-fixation depends upon

    • cultivar

    • Number of nodules

    • Efficient strain of bacteria existing in their root nodules


Azotobacter
Azotobacter symbiotic

  • Free lioving

  • Aerobic organisms

  • Nitrogen fixer

  • Affect on seed growth

  • Bind atmosphric N and release ammonium ion into soil

  • Growth promoter


Azospirillum
Azospirillum symbiotic

  • General root colonizer

  • Spiral shaped

  • Produce hormones and vitamines

  • Fix the nitrogen

  • Provide nutritions to cereal crops


Bacillus
bacillus symbiotic

  • Most abundant geneus in rhizosphere

  • Activity as PGPR known for many years

  • Release number of meatabolites

  • P-solublizers

  • N-fixer


Pseudomonas
Pseudomonas symbiotic

  • Ubiquitous bacteria

  • Help in maintenance of soil health

  • P-solublizers

  • N-fixer


Methodology
METHODOLOGY symbiotic

  • Isolation of Az, As, Ps, Bc sp were isolated by standard dilution method.

  • Chick pea plants uprooted

  • Nodules separated and sterilized

  • Obtain suspension

  • Streaked on Congo red YMA medium

  • Colorless, purified colonies picked and re-streaked to obtain pure culture

  • Purified rhizobial culture, streaked on slants


Yma medium
YMA medium symbiotic


Pot study
Pot study symbiotic

  • Complete Randomized Design (CRD) with six treatments and three replications.

  • T1=Control

  • T2=Azotobacter sp

  • T3=Azospirillum sp

  • T4 =Rhizobium sp (chick pea)

  • T5 = Pseudomonas sp

  • T6 = Bacillus sp


Results
Results symbiotic

  • Effect of treatments on root and shoot parameters of maize.



Conclusion
conclusion mass of maize

  • Clearly indicated that PGPR

  • Influenced the groeyh of maize considerably

  • Az show highest ABP and after it Rhizobium CP

  • Bacillus shows better growth of root, shoot parameters as compared to others


Isolation of rhizobium
ISOLATION OF RHIZOBIUM mass of maize

Pink Healthy Nodules

Washing

Sterilization (95% Ehanol)

Washing

0.1% HgCl2

Washing

Crushing

Streaking on YMA Petri Plate


Isolation of free livings
ISOLATION OF FREE LIVINGS mass of maize

  • Dilution Plate Technique


Results1
RESULTS mass of maize

  • isolations of different microbes


Soil analysis
SOIL ANALYSIS mass of maize

  • Different soil samples were analyzed for:

  • Organic matter (walkley black method)

  • Phosphorus (Olsen and sommers method)


Results table determination of available organic matter from different soil sample
Results mass of maizeTable: Determination of available organic matter from different soil sample


Results table determination of available phosphorous from different soil sample
Results mass of maizeTable: Determination of available Phosphorous from different soil sample


Thanks

thanks mass of maize


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