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Enumeration. GoalTo determine the number of bacteria per milliliter of sampleCells/mlMethodsDirectStaining and counting under microscopeHeterotrophic plate countCount colonies and assume each colony represents 1 cell in original cultureIndirect: estimate based on another propertyMPNStatistical estimate based on growth patterns in mediaSpectroscopyEstimate based on turbidity (or transmittance of light).

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