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Practical Tips of ELISA

Enzyme-linked Immunosorbent Assays (ELISAs) combine the specificity of antibodies

with the sensitivity of simple enzyme assays, by using antibodies or antigens coupled

to an easily-assayed enzyme. ELISAs can provide a useful measurement of antigen or

antibody concentration. Being one of the most sensitive immunoassays, ELISA offers

commercial value in laboratory research, diagnostic of disease biomarkers, and

quality control in various industries.

There are different types of ELISA according to the analyte detection method. Each of

them have its own protocol but the basic principle is similar. An ELISA is a generally a

five-step procedure:

1) Antigen coating;

2) Blocking all unbound sites to prevent non-specific absorption;

3) Add analyte and incubation;

4) Add labeled antibody and incubation;

5) Add reagents for colouration/ luminescence, thus give a positive result.

We have already discussed the ELISA format and general protocol in ELISA Guide and

ELISA Development Guide. Here we mainly discuss about some basic knowledge and

practical tips during the operation process of ELISA. If you need more information

about commercial ELISA kit, please visit our ELISA Kit Product.

Creative Diagnostics provides practical tips of elisa for your research.

http://www.creative-diagnostics.com/Practical-Tips-of-ELISA.htm

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