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Searching the NCBI Databases

Searching the NCBI Databases. Search for CFTR[gene] in the Protein database. What is it?. Limit to genes in human only CFTR[gene] AND human[orgn]. How many?. Restrict to RefSeq. How many?. RefSeq records can also be filtered using the Limits tab. Examine the NP_000483 record

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Searching the NCBI Databases

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  1. Searching the NCBI Databases

  2. Search for CFTR[gene] in the Protein database What is it?

  3. Limit to genes in human onlyCFTR[gene] AND human[orgn] How many?

  4. Restrict to RefSeq How many?

  5. RefSeq records can also be filtered using the Limits tab

  6. Examine the NP_000483 record How many amino-acids are in the protein? On which chromosome is this protein-coding gene located?

  7. Follow the dbSNP link to examine the SNPs associated with this protein How many SNPs?

  8. Follow the dbSNP link to examine the SNPs associated with this protein Describe mutation in first record. Is it synonymous?

  9. Use the Taxonomy Browser to examine fully-sequenced primates genomes

  10. Use the Taxonomy Browser to examine fully-sequenced prototheria genomes

  11. Sequence Retrieval

  12. Search for human BRCA1 in Protein database Select longest isoform

  13. Display the sequences in FASTA format

  14. Save the sequence into a WORD file

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