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871602 Shishi’s seminar 2000,11,16

The Human Tap Nuclear RNA Factor Contains a Novel Transportin-dependent Nuclear Localization Signal That Lacks Nuclear Export Signal Function Ray Truant, Yibin Kang, and Bryan R. Cullen. 871602 Shishi’s seminar 2000,11,16. Background:

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871602 Shishi’s seminar 2000,11,16

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  1. The Human Tap Nuclear RNA Factor Contains a Novel Transportin-dependent Nuclear Localization Signal That Lacks Nuclear Export Signal FunctionRay Truant, Yibin Kang, and Bryan R. Cullen 871602 Shishi’s seminar 2000,11,16

  2. Background: ‧ At least two possible protein mediators of mRNA export exist: heterogeneous nuclear ribonucleoproteins (hnRNPs) and Tap protein. ‧ Definitions of importinαβ , importin β binding domain (IBB) , nuclear localization signal (NLS) , nuclear export signal (NES) , transportin (Trn) , hnRNP A1 and M9 . ‧ M9: NLS/NES Tap-N: NLS

  3. Background: ‧ At least two possible protein mediators of mRNA export exist: heterogeneous nuclear ribonucleoproteins (hnRNPs) and Tap protein. ‧ Definitions of importinαβ , importin β binding domain (IBB) , nuclear localization signal (NLS) , nuclear export signal (NES) , transportin (Trn) , hnRNP A1 and M9 . ‧ M9: NLS/NES Tap-N: NLS

  4. ‧ Functional domain organization of human Tap

  5. Transportin is necessary and sufficient for Tap-N-NLS-dependent nuclear import. ‧Competitive nuclear import assays.

  6. ‧ Transportin is sufficient for Tap-N-NLS-dependent nuclear import.

  7. Direct binding of the Tap-N-NLS by Trn ‧In vitro binding assays.

  8. ‧Nuclear uptake of the Tap-N-NLS is sequence-specific.

  9. Functional comparison of M9 and Tap-N-NLS ‧ Heterokaryon fusion nuclear shuttling assay.

  10. ‧HeLa cell microinjection assays.

  11. Three types of Trn-dependent protein NLS sequences. ‧M9: specific for Trn , NLS/NES . ‧Ribosomal proteins: can also be mediate by Impβ ,NLS , no homology to M9. ‧Tap: no homology to M9 , but specific for Trn , compete with M9 , but lacks NES . Trn nuclear import factor may have evolved a specialized role to the return of mRNA export factors to the nucleus.

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