Synthetic Biology - Progess in Merging Nanoparticles and Proteins
This presentation is the property of its rightful owner.
Sponsored Links
1 / 6

Synthetic Biology - Progess in Merging Nanoparticles and Proteins PowerPoint PPT Presentation


  • 41 Views
  • Uploaded on
  • Presentation posted in: General

Synthetic Biology - Progess in Merging Nanoparticles and Proteins. Kie Moon Sung David Mosley Joseph Jacobson Center for Bits and Atoms, MIT Beau Peele Shuguang Zhang Biomedical Engineering Dept, MIT. Nanoparticle Building Blocks. COOH. COOH. NH 2. NH 2. COOH. COOH. NH 2. NH 2.

Download Presentation

Synthetic Biology - Progess in Merging Nanoparticles and Proteins

An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -

Presentation Transcript


Synthetic biology progess in merging nanoparticles and proteins

Synthetic Biology - Progess in Merging Nanoparticles and Proteins

Kie Moon Sung

David Mosley

Joseph Jacobson

Center for Bits and Atoms, MIT

Beau Peele

Shuguang Zhang

Biomedical Engineering Dept, MIT


Synthetic biology progess in merging nanoparticles and proteins

Nanoparticle Building Blocks

COOH

COOH

NH2

NH2

COOH

COOH

NH2

NH2

COOH

NH2

Natural amino acids

Amino acid-nanoparticles


Synthetic biology progess in merging nanoparticles and proteins

Synthesis of Lys-Monofunctionalized Gold Nanocrystals

aReagents and conditions: (a) H2NNH2·H2O/DMF (2% v/v), DIPEA/DMF (10% v/v). (b) HS(CH2)10CO2H (excess), THF. (c) DIC (excess), DMF/THF (10% v/v) rt, 24h. (d) MeNH2, DIC (excess), DMF/THF, rt, 12h. (e) 60% TFA, 2.5% TIS, 2.5% H2O, 35% DMF, 24h.


Synthetic biology progess in merging nanoparticles and proteins

Identification of Monofunctionalized Nanocrystals

Nanocrystal Dimers

10 nm

At least 60% of Nanocrystals are monofunctionalized.

J.Am.Chem. Soc., 126 (2004), p. 5064


Synthetic biology progess in merging nanoparticles and proteins

Programmable Assembly of Nanocrystal-Peptides

Fmoc SPPS Protocols

peptide1

peptide2

peptide3


Synthetic biology progess in merging nanoparticles and proteins

Ultimate Goals

  • New Structures - New peptide-folding rules governed by nanoparticles.

  • New Functions – Electronic control of specific protein or enzyme functions, electronic feedback aka new biosensor techniques.

  • New materials – Both bioinorganics and a new class of nanoparticle polymers.

All aided by the programmable assembly nature of this building block based technology. This concept will allow us to access completely new motifs, and readily explore the phase space.


  • Login