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Anaerobic Culture Methods. Reducing media Contain chemicals (thioglycollate or oxyrase) that combine O 2 Heated to drive off O 2. Anaerobic Culture Methods. Anaerobic jar. Figure 6.5. Anaerobic Culture Methods. Anaerobic chamber. Figure 6.6. Capnophiles Require High CO 2. Candle jar

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anaerobic culture methods
Anaerobic Culture Methods
  • Reducing media
    • Contain chemicals (thioglycollate or oxyrase) that combine O2
    • Heated to drive off O2
anaerobic culture methods1
Anaerobic Culture Methods
  • Anaerobic jar

Figure 6.5

anaerobic culture methods2
Anaerobic Culture Methods
  • Anaerobic chamber

Figure 6.6

capnophiles require high co 2
Capnophiles Require High CO2
  • Candle jar
  • CO2-packet

Figure 6.7

selective media
Selective Media
  • Suppress unwanted microbes and encourage desired microbes.

Figure 6.9b–c

differential media
Differential Media
  • Make it easy to distinguish colonies of different microbes.

Figure 6.9a

enrichment media
Enrichment Media
  • Encourages growth of desired microbe
  • Assume a soil sample contains a few phenol-degrading bacteria and thousands of other bacteria
    • Inoculate phenol-containing culture medium with the soil and incubate
    • Transfer 1 ml to another flask of the phenol medium and incubate
    • Transfer 1 ml to another flask of the phenol medium and incubate
    • Only phenol-metabolizing bacteria will be growing
slide8
A pure culture contains only one species or strain.
  • A colony is a population of cells arising from a single cell or spore or from a group of attached cells.
  • A colony is often called a colony-forming unit (CFU).
streak plate
Streak Plate

Figure 6.10a–b

preserving bacteria cultures
Preserving Bacteria Cultures
  • Deep-freezing: –50°to –95°C
  • Lyophilization (freeze-drying): Frozen (–54° to –72°C) and dehydrated in a vacuum
reproduction in prokaryotes
Reproduction in Prokaryotes
  • Binary fission
  • Budding
  • Conidiospores (actinomycetes)
  • Fragmentation of filaments
binary fission
Binary Fission

Figure 6.11

direct measurements of microbial growth
Direct Measurements of Microbial Growth
  • Plate counts: Perform serial dilutions of a sample

Figure 6.15, step 1

plate count
Plate Count
  • Inoculate Petri plates from serial dilutions

Figure 6.16

plate count1
Plate Count
  • After incubation, count colonies on plates that have 25-250 colonies (CFUs)

Figure 6.15

direct measurements of microbial growth2
Direct Measurements of Microbial Growth
  • Multiple tube MPN test.
  • Count positive tubes and compare to statistical MPN table.

Figure 6.18b

measuring microbial growth
Direct methods

Plate counts

Filtration

MPN

Direct microscopic count

Dry weight

Indirect methods

Turbidity

Metabolic activity

Dry weight

Measuring Microbial Growth
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