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Anaerobic Culture Methods. Reducing media Contain chemicals (thioglycollate or oxyrase) that combine O 2 Heated to drive off O 2. Anaerobic Culture Methods. Anaerobic jar. Figure 6.5. Anaerobic Culture Methods. Anaerobic chamber. Figure 6.6. Capnophiles Require High CO 2. Candle jar

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Anaerobic culture methods
Anaerobic Culture Methods

  • Reducing media

    • Contain chemicals (thioglycollate or oxyrase) that combine O2

    • Heated to drive off O2


Anaerobic culture methods1
Anaerobic Culture Methods

  • Anaerobic jar

Figure 6.5


Anaerobic culture methods2
Anaerobic Culture Methods

  • Anaerobic chamber

Figure 6.6


Capnophiles require high co 2
Capnophiles Require High CO2

  • Candle jar

  • CO2-packet

Figure 6.7


Selective media
Selective Media

  • Suppress unwanted microbes and encourage desired microbes.

Figure 6.9b–c


Differential media
Differential Media

  • Make it easy to distinguish colonies of different microbes.

Figure 6.9a


Enrichment media
Enrichment Media

  • Encourages growth of desired microbe

  • Assume a soil sample contains a few phenol-degrading bacteria and thousands of other bacteria

    • Inoculate phenol-containing culture medium with the soil and incubate

    • Transfer 1 ml to another flask of the phenol medium and incubate

    • Transfer 1 ml to another flask of the phenol medium and incubate

    • Only phenol-metabolizing bacteria will be growing



Streak plate
Streak Plate

Figure 6.10a–b


Preserving bacteria cultures
Preserving Bacteria Cultures

  • Deep-freezing: –50°to –95°C

  • Lyophilization (freeze-drying): Frozen (–54° to –72°C) and dehydrated in a vacuum


Reproduction in prokaryotes
Reproduction in Prokaryotes

  • Binary fission

  • Budding

  • Conidiospores (actinomycetes)

  • Fragmentation of filaments


Binary fission
Binary Fission

Figure 6.11






Direct measurements of microbial growth
Direct Measurements of Microbial Growth

  • Plate counts: Perform serial dilutions of a sample

Figure 6.15, step 1


Plate count
Plate Count

  • Inoculate Petri plates from serial dilutions

Figure 6.16


Plate count1
Plate Count

  • After incubation, count colonies on plates that have 25-250 colonies (CFUs)

Figure 6.15



Direct measurements of microbial growth2
Direct Measurements of Microbial Growth

  • Multiple tube MPN test.

  • Count positive tubes and compare to statistical MPN table.

Figure 6.18b


Direct measurements of microbial growth3
Direct Measurements of Microbial Growth

  • Direct microscopic count



Estimating bacterial numbers by indirect methods
Estimating Bacterial Numbers by Indirect Methods

  • Turbidity

Figure 6.20


Measuring microbial growth

Direct methods

Plate counts

Filtration

MPN

Direct microscopic count

Dry weight

Indirect methods

Turbidity

Metabolic activity

Dry weight

Measuring Microbial Growth


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