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Ministry of Health and Social Development of the Russian Federation State Educational Institution Of Higher Professional Education Saratov State Medical University named after V.I. Razumovsky.
State Educational Institution Of Higher Professional Education
Saratov State Medical University named after V.I. Razumovsky
Morphological changes in the inner organs and tumor of laboratory animals after administration of Fenanoparticles
Saratov State Medical University n.a. V.I. Razumovsky
N.A. Navolokin, A.B. Bucharskya, G.N. Maslyakova,
Saratov State University
D.A. Gorin D.A., S.V. German
to investigate the morphological changes in organs and transplanted tumors of laboratory animals with parenteral methods of administration Fe nanoparticles.
White outbred half-year-old male rats inoculated with liver cancer PC-1, citrate coated iron nanoparticles (Fe3O4, 20 nm ± 10), Zp = -30 mv, were used in the study. Animal experiments were performed in accordance with the guidance «International Guiding principles for Biomedical Research Involving Animals» (2012). Animals with tumor transplanted liver cancer PC-1 were divided into 3 groups (10 rats were in each group). In the first group the nanoparticle solution was administered once intravenously at a dosage of 0.2 mg / kg; in the second group the nanoparticle solution was administered in a dosage of 16 mg/kg. The third group (comparison group) received a single intravenous injection of 1 ml saline.
The increase of liver and kidney weight was observed macroscopically after administration of Fe nanoparticles, due to the higher degree of venous congestion, the development of vascular and degenerative processes in hepatocytes and epithelial cells of the convoluted tubules. Dystrophic and necrotic processes may depend on disturbance of blood circulation due circulation of iron nanoparticles in the lumen of blood vessels, and due to the toxic influence of the tumor itself . Accumulations of iron nanoparticles and hemosiderin were not found by this method and the duration of administration.
Statistically significant thinning of interalveolar septa and emphysema with increasing blood supply vessels were occured in the lungs. Clusters of nanoparticles were not revealed, but a significant increase in the area of peribronchial lymphoid infiltrates was noted, which indicates body’s immune response to the introduction of foreign particles.
The intravenous administration of citrate - stabilized iron nanoparticles in a dosage of 20 mkg/kg leads to disruption of blood supply to the organs, mainly due to the plethora, while marked dystrophic cell damage is observed in two main detoxification organs: in the liver and kidneys. The immunostimulatory effects of iron on the white pulp of the spleen and peribronchial lymphoid follicles were established. Particles were detected in the heart and brain, and their accumulation in the tumor was significantly noted. This data indicates that the selected dose is sufficient for accumulation of the iron nanoparticles in tumor.