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p 450 hits against 454 database

p 450 hits against 454 database. Isolate the sequence from scaffolds. >scaffold02252| 4452:4790 |CYP51G2|2e-40

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p 450 hits against 454 database

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  1. p450 hits against 454 database

  2. Isolate the sequence from scaffolds >scaffold02252|4452:4790|CYP51G2|2e-40 ACGTGGCCGGTCTTCGGCGGGTTAATCCGGTTCctAAAAGGaCCGGTTGTGATGCTAAGAGAAGAGTACCCGAAACTAGGGAGTGTGTTCACGTTGAACTTGTTCAACAAAAAGATCACTTTCTTTATCGGGCCTGAGGTTTCAGTCCATTTCTTTAAAGCTTCTGAATCTAATCTGAGCCAACAAGAAGTGTACAAGTACATTGTGCCTACCTTTGGGCCCGGAGTGATTTACGATGTTGATTATAGCGTACGGCAGGAGCAGTTTCGGTTCTTTACTGAGAGTCTAAGGGTTAACAAGTTGAAAGGTTATGTGGGTCAGATGCTTATTGAAGCTCA >scaffold02252|8063:8362|CYP51G2|8e-35 TCGCCTGCTTTCGCTAATCgTCTCCCTCATATATTCAAGGATCCGGACACGTTTGACCCTGACAGATTTGCTCCCGGTAGGGAagAAGACAAAGTGTCTGGGGCgTTttCGTACATCTCTTTTGGCGGTGGCAGGCACGCGTGTCTAGGGGAGCCGTTCGCGTATCTGCAAATAAAATCgATTTGGAGCCATTTGCTGAGGAACTTTGAGCTGGAACTGGTTTCACCTTTTCCGGAGATTGACTGGAATGCCATGGTCGTGGGTGTGAAAGGAAaGgTTATGGTGCGGTACAAGCGCCG >scaffold06866|14030:14566|CYP51G2|6e-16 ACTGAATCTTTTTGTACTTCCAAATTACAGGACATGTTtGTGGGAGGAaCTGATACAACCTCAGCAACCATGGAGTGGGCAATGGCAGAGCTGATGAAAAaTCCAACAAaGATgAaGAAAGCTCAAAAaGAGGTGCGAAGAGTGGTTGGGAAAAAaTCCAAGAtcAGTCAAGATGATATTGCTCAAATGGAATACTTAAAaTGTGTTGTCAAAGAgACTCTTAGATTACATCCTCCAGCTCCTTTTTtGGTtCCTAGAGAATTGACTGAGAGTACTACATTGGAAGGCTATGATATTCCTCCAAAAaCAAGAATATTCATTAATGCATGGGCAATTCAAAGagATTCCAAATACTGGGACCAGCCAGAAGAATTCcTTCCAGAGAGATTtGCTAACAACTTGGTTGATTTtAGAGGCCAAGAcTTCCAaTTCATTCCATTTGGAGCAGGGCgAAGGGGCTGTCCTGGCATTTCATTTGGTGTCATGGAAGTTGAACTTGTACTTGCCAACCTCTTGTACTGGTTTGACTGGAAGTT >scaffold06866|9256:9750|CYP51G2|6e-15 GCAGGAACTGATACAACTTCAACAACCTtAGAGTGGGCAATGGCAGAGCTAATGAAAAGTCCAACTAGGATGAAGAAAaTtCAAGAaGAGGTACGAAGaGTGGTtGGgAAGAAaTCCAAGATCAATCAAgaTGATATtGATCAAATGgACTACTTAAAATGTGTTGTCAAAGAGACTCTTAGATTACATCCTCCAGGTCCTTTTTtGGTtCCTAGAGAATCAAGTGAGAGTACTAAATTGGGAGGTTATGATATTCCTCCAAAAACAAGAATTTTCATCAATGTTTGGGCAATTCAAAGAGATCCCAAATACTGGGATAGGCCCGAAGAATTTCTTCCAGAGAGATTTGCTAACAACCCGATTGATTTtAAAGGCAAAGACTTCGAATTCATCCCATTTGGTGCAGGGCGAAGGGGCTGTCCTGGAATTTCATTTGCAATCACTGAAACTGAACAAGTGCTTGCCAATCTCTTGTACTGGTTTGATTGGAAGTT

  3. Align isolated sequences with EST >scaffold02252|4452:4790|CYP51G2|2e-40 ACGTGGCCGGTCTTCGGCGGGTTAATCCGGTTCctAAAAGGaCCGGTTGTGATGCTAAGAGAAGAGTACCCGAAACTAGGGAGTGTGTTCACGTTGAACTTGTTCAACAAAAAGATCACTTTCTTTATCGGGCCTGAGGTTTCAGTCCATTTCTTTAAAGCTTCTGAATCTAATCTGAGCCAACAAGAAGTGTACAAGTACATTGTGCCTACCTTTGGGCCCGGAGTGATTTACGATGTTGATTATAGCGTACGGCAGGAGCAGTTTCGGTTCTTTACTGAGAGTCTAAGGGTTAACAAGTTGAAAGGTTATGTGGGTCAGATGCTTATTGAAGCTCA Query= scaffold02252|8063:8362|CYP51G2|8e-35 > Contig9662 Query 4 CCTGCTTTCGCTAATCGTCTCCCTCATATATTCAAGGATCCGGACACGTTTGACCCTGAC 63 ||||||||||||||||||||||||||||| |||||||||||||||||||||||||||||| Sbjct 582 CCTGCTTTCGCTAATCGTCTCCCTCATATTTTCAAGGATCCGGACACGTTTGACCCTGAC 641 Query 64 AGATTTGCTCCCGGTAGGGAAGAAGACAAAGT-GTCTGGGGCGTTTTCGTACATCTCTTT 122 ||||||||||||||||| |||||||||||||| | ||||||| ||||||||||||||||| Sbjct 642 AGATTTGCTCCCGGTAGAGAAGAAGACAAAGTAG-CTGGGGCATTTTCGTACATCTCTTT 700 Query 123 TGGCGGTGGCAGGCACGCGTGTCTAGGGGAGCCGTTCGCGTATCTGCAAATAAAATCGAT 182 ||| ||||||||||||| |||| |||||||||| || || ||||||||||||||| | || Sbjct 701 TGGTGGTGGCAGGCACGGGTGTTTAGGGGAGCCTTTTGCTTATCTGCAAATAAAAGCCAT 760 Query 183 TTGGAGCCATTTGCTGAGGAACTTTGAGCTGGAACTGGTTTCACCTTTTCCGGAGATTGA 242 ||||||||||||||||||||| ||||||||||||||||| |||||||||||||||||||| Sbjct 761 TTGGAGCCATTTGCTGAGGAATTTTGAGCTGGAACTGGTCTCACCTTTTCCGGAGATTGA 820 Query 243 CTGGAATGCCATGGTCGTGGGTGTGAAAGGAAAGGTTATGGTGCGGTACAAGCG 296 |||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 821 CTGGAATGCCATGGTCGTGGGTGTGAAAGGAAAGGTTATGGTGCGGTACAAGCG 874

  4. Issues/Frustrations • >scaffold07086|5306:4950|CYP51G2|6e-45 • Could not find good software… • JCVI, Washington University Genome Center • Blast has “tool” to isolate… poor documentation • Artemis stinks

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