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FZD7 Supplementary figures. Supplementary Figure 1. A). SKOV3-P 2D Parental. SKOV3-S 3D Spheroid. SKOV3-S2D Spheroid-reattached. B). Supplementary Figure 2. FZD7-6 siRNA. FZD7-5 siRNA. NEG. CONTROL. A). CH1. PA-1. OV-17R. PI. Annexin-V. Supplementary Figure 2. ANNEXIN V. B).

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Presentation Transcript
slide2

Supplementary Figure 1

A)

SKOV3-P

2D Parental

SKOV3-S

3D Spheroid

SKOV3-S2D

Spheroid-reattached

B)

slide3

Supplementary Figure 2

FZD7-6 siRNA

FZD7-5 siRNA

NEG. CONTROL

A)

CH1

PA-1

OV-17R

PI

Annexin-V

slide4

Supplementary Figure 2

ANNEXIN V

B)

C)

D)

slide6

Supplementary Figure 4

b-cat

F-actin

Merge

CH1

b-cat

F-actin

Merge

PA-1

b-cat

F-actin

Merge

OV-17R

NEG. CONTROL

FZD7-6

FZD7-5

slide7

Supplementary Figure 5

CH1

PA-1

OV-17R

NEG. CONTROL

FZD7-6

FZD7-5

slide9

Supplementary Figure 7

A) CH1

0-hrs

15-hrs

FZD7-5 siRNA

FZD7-6 siRNA

NEG. CONTROL

slide10

Supplementary Figure

B) PA-1

0-hrs

15-hrs

FZD7-5 siRNA

FZD7-6 siRNA

NEG. CONTROL

slide11

Supplementary Figure

C) OV-17R

0-hrs

32-hrs

FZD7-5 siRNA

FZD7-6 siRNA

NEG. CONTROL

slide12

Supplementary Figure 8

A) CH1

Merge

γ-tubulin

Ck1ε

Merge

γ-tubulin

DVL2 pSer143

NEG.

CONTROL

Merge

γ-tubulin

Ck1ε

Merge

γ-tubulin

DVL2 pSer143

FZD7-5

Merge

γ-tubulin

Ck1ε

Merge

γ-tubulin

DVL2 pSer143

FZD7-6

slide13

Supplementary Figure 8

B) PA-1

Merge

γ-tubulin

DVL2 pSer143

Merge

γ-tubulin

Ck1ε

NEG.

CONTROL

γ-tubulin

DVL2 pSer143

Merge

γ-tubulin

Ck1ε

Merge

FZD7-5

Merge

γ-tubulin

DVL2 pSer143

Merge

γ-tubulin

Ck1ε

FZD7-6

slide14

Supplementary Figure 8

C) OV-17R

Merge

γ-tubulin

Ck1ε

Merge

γ-tubulin

DVL2 pSer143

NEG.

CONTROL

Merge

γ-tubulin

Ck1ε

Merge

γ-tubulin

DVL2 pSer143

FZD7-5

Merge

γ-tubulin

Ck1ε

Merge

γ-tubulin

DVL2 pSer143

FZD7-6

slide15

Supplementary Figure 9

FZD7-5 siRNA

FZD7-6 siRNA

FZD7-6 siRNA

FZD7-5 siRNA

+Wnt5a

+Wnt5a

+Wnt5a

+Wnt5a

B)PA1

A)CH1

-Wnt5a

-Wnt5a

-Wnt5a

-Wnt5a

+Wnt5a

+Wnt5a

C)OV-17R

-Wnt5a

-Wnt5a

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