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kin17. Novel protein related to splicing/mRNA processing, transcription, and cell cycle regulation . Chromosomal proteins often interact with DNA to control maintenance, propagation, and expression of the genome.

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kin17

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kin17

  • Novel protein related to splicing/mRNA processing, transcription, and cellcycleregulation.

  • Chromosomal proteins often interact with DNA to control maintenance, propagation, and expression of the genome.

  • Kin17 forms intranuclear foci and accumulates in the nuclei of proliferating cells.

  • Cells presenting low levels of this protein also showed a prolongation of the S phase of the cell cycle associated with an accumulation of cells in early and mid-S phase, a decreased rate of DNA synthesis, and an increased sensitivity to gamma irradiation.


Ultrastructuralcolocalization of kin17 protein with DNA replication proteins.

kin17/RPA70

kin17/PCNA

Human RKO cells

kin17/DNA polymeraseα


kin17 protein is a component of high-molecular-weight nuclear protein complexes.


kin17 protein is a component of the MRC in human HeLa cells.

suggesting that the kin17 protein might be a part of the MRC

purifiedbyMono-Q chromatography


kin17 protein copurifies with an RC complex from S-phase HeLa cells


kin17 protein copurifies with an RC complex from S-phase HeLa cells

669 kDa RC complex


Immunodepletion of kin17 protein inhibits in vitro replication of SV40 origin- and large T antigen-dependent DNA replication


Direct involvement of kin17 protein in DNA replication

Human

Monkey


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