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BRUCELLOSIS by. HASUTJI ENDAH NARUMI. DEPARTMENT OF MICROBIOLOGY; LAB OF BACTERIOLOGY FACULTY OF VETERINARY MEDICINE AIRLANGGA UNIVERSITY. PRIMER attack to cow, sheep and pig. SECUNDAIRattack to other animals and human being. COWBang’s disease.

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Brucellosis by

BRUCELLOSIS

by

HASUTJI ENDAH NARUMI

DEPARTMENT OF MICROBIOLOGY; LAB OF BACTERIOLOGY

FACULTY OF VETERINARY MEDICINE

AIRLANGGA UNIVERSITY


Brucellosis by

PRIMER attack to cow, sheep and pig

SECUNDAIRattack to other animals

and human being

COWBang’s disease

HUMANundulans fever (malta fever)

Brucellosis = Abortus Infections Disease


Brucellosis by

ECONOMIC COST

  • Abortus

  • Give birth to meal

  • Reproduction disturbance infertility

  • Milk product x

  • Low mortality

  • Treatment value

  • Zoonosis (important)


Brucellosis by

ETIOLOGY

  • -Stick

    -Gram (-)

    -Non motile

    -Non spora

  • Intra seluler

  • Three species which attack to livestock

    - B. militensis

    - B. abortus

    - B. suis


Brucellosis by

  • Cow and dogB. abortus infections abortus

  • B. abortus attack horses, pigs

  • B. suis pigs, dogs

  • B. melitensis sheeps

EPIZOOTIOLOGY

Disseminating cow milk in Java

Susceptible animal

Rescue institute of animal disease

Bekasi, Bogor, Kediri & Jakarta Health office

human which work at Denpasar


Brucellosis by

PATHOGENESIS

The transmission of disease

Through

  • Gastrointestinal tract

  • Genitalia tract

  • Skin, mucosa


Brucellosis by

  • Infectious abortus temporary / permanent of 5 - 8 months

  • Occur revers to normal

  • milk product

  • Health produce infectious vaginal liquor

  • Mindness of foetal liquor

  • Pituitary gland ≠ symptom, other side milk contaminated againts bacteri nipple zoonosis

  • Epidydimitis (Male Epidydimitis) & orchitis

SYMPTOM OF THE DISEASE

Clinical symptom


Brucellosis by

2. POST MORTEM

  • Thickening of placenta

  • Spreads over the chorions layers

  • Mindness of foetal of liquor, brown - yellow, mixed with pus


Brucellosis by

Male : Testis Inppurate Necrose

Removal and Materials Delivery

  • Put into ice (suggestion)

  • Delivery to the lab

  • Put on preserved solution

  • Materials :

  • Milk : all nipple / not all nipples contaminated with bacteria

  • Vol 20 ml < washing with alc sol 70%

  • 1st, 2nd out pouring should be throw among

  • In the cold condition


Brucellosis by

Vagina : removal on 6 weeks

post partum / abortus

Using wire, with cotton point (absorb a thread) put the cotton in to the glass jar

Blood cultivate (from V. Jugularis)

- This area should be wash and rubbed this area using cotton talk 70%.

-Put 10 ml with spuit + heparin (anti coagulant)

Blood serologic test


Brucellosis by

Placenta : infections cotyledone

smears slide fictations using methanol sol

Fetus abortus :

-Disposal of gatric

- Pulmo

- Lien

- Fetal disposal

- Take with sterile pasteur pipet

Carcass :

-The predict of bact is different among the species

animals

-Cow lgl supramamaria, lien, nipple, uterus


Brucellosis by

DIAGNOSIS

- Material samples

-Base on :

  • Clinical symptom

  • PA

  • Laboratory :

    - Isolated and bacteria identification

    - Serologic test, blood serum thrid of

    mucosa vagina / milk, sperm


Brucellosis by

DIFFRENTIAL DIAGNOSA

  • Abortus:infection camphylobacter

    foetus and trichomonas foctos

  • C. Foetusabortus usually happen

    every time

  • T. Foetusabortus happen earhide

  • Brucellaabortus happen after

    6 months


Brucellosis by

PREVENTIVE OF THE DISEASE

Route of administration :

  • to classified among

  • report the sample

  • to give the certificate for free Brucellosis

  • to give a tag for each sample post vacc & reactor


Brucellosis by

Sanitation :

  • To burn the infectious material

  • To avoid a matting which suffer from a disease

  • Avoid for young animals from mother which suffer from a disease to give milk

  • Crages should be disinfected to avoid suffer from a disease

  • To refreshment a animals should be free from brucellosis

  • To monitor the mobilitation or animals


Brucellosis by

ERADICATION

  • Standardization Dx / for Brucell (methods and reagent)

  • To point out infect and healthy area from Brucellosis

  • To point out infections cattles and healthy area from brucella

  • To point a policy for use a vaccination

  • To give a certificate of health for a healthy care


Brucellosis by

Leptospirosis = Weil’s disease

  • Attacks animals and human

  • Cause of spirochaeta bact, genus of leptospira

  • The change subclinic of infectious were : abortion, hepatitis and nephritis death


Brucellosis by

  • Resistant inside the water (1 month)

  • Inside the sea water, urine + rapidly

ETHIOLOGY

F o r m :

  • Long filament

  • Gram (-)

  • Aquatic saprofite apparently


Brucellosis by

  • Isolation from human, dog, pig and micer / rat

  • Serolog reactor + cow, buffalo and pig

  • Pig at RPH Bogor, which contain bacteria inside the ren

  • At middle & East Java economic cost at the cattle of pig and cow

EPIZOOTIOLOGI

Incident in Indonesia


Incident in philippine

Incident in Philippine


Brucellosis by

The Familiar Of Several Leptospira

  • Leptospira canicola dog

  • Leptospira harjo cow

  • Leptospira ictero haemorrhagie mice / rat

  • Human infection through water, soil, flora which contaminated of animal urine

  • Incubation periods 4 - 19 days


Brucellosis by

PATOGENESIS

The Route of Disease Mechanisms

  • Bact body through mucosal layer multiple especially in : liver, ren, mammary gland, brain layer

  • Leptospiremia 1st week post infect

  • Bact produce toxin and hemolysin

    blood capilary disturbance

    tissue disturbances

  • Bact grow at tubulus renalis release via urine

  • Bact still alive in the urine several hour at 150C (several days at 40C)

  • Lethal because of septichemia, anemia, liver disorder and uremia

  • State of infect depend on :age, species, bact reservoir

    cause of infect.


Brucellosis by

Human Infected From Contaiminated Animals (Rats)

Bact inside the ren come out from urine

  • Cow (acute, subacute and chronic)

  • Acute : »fever, anemia, T after 2 - 4 days

  • »Milk prod become normal after 2 weeks

  • »Milk produce mixed with pus

  • Pig : »abortus

  • »retentio secundinarum

  • »+ neonatal

  • »bact vehicle for cow & human

CLINICAL SYMPTOM


Brucellosis by

POSTMORTEM DISORDER

  • Cow : liver abses, ren colored

    black dark

  • Calf : icterus, liver absces, dark

    ren, petechial haemorrhagie,

    VU contain clean red urine

  • Chronic :absces ren, if slices contained

    of liquid


Brucellosis by

DIAGNOSIS

  • Bacterial Isolation

  • Inject animal

  • Live animal under fever condition :

    -Urin without preserve

    -Aseptic blood + citrate

    -Aseptic milk and not preservative

  • From tetal animal

    -Urine, liver slices, ren, lien and blood cor

  • From healthy animals

    -Urine and serum

Material of Sample


Brucellosis by

  • Babesiosis with blood strike not effective againt of leptospirosis

  • Because the symtomp is abortus until confused with the cause of the other abortus

  • Clinical symptom, Hb uria, cause of clostridum, haemolyticum

DIFFERENSIAL DIAGNOSIS


Brucellosis by

PREVENTION

  • Death of animal - which bring the leptospira especially rodentia mild

  • R/ Antibiotics (Penicilline) L. canicola

    - Streptomycine, Aureomycine


Thank you

THANK YOU


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