Valorization of glycerol through the production of biopolymers: The PHB
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Valorization of glycerol through the production of biopolymers: The PHB case using Bacillus megaterium. Javier M. Naranjo , John A. Posada, Juan C. Higuita , Carlos A. Cardona Instituto de Biotecnología y Agroindustria, Universidad Nacional de Colombia sede Manizales, .

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Valorization of glycerol through the production of biopolymers: The PHB case using Bacillus megaterium

Javier M. Naranjo, John A. Posada, Juan C. Higuita, Carlos A. Cardona

Instituto de Biotecnología y Agroindustria, Universidad Nacional de Colombia sede Manizales,

Betreuer: Benjamen Stenger

Ammar Abdulmughni


Overview
Overview biopolymers: The PHB

  • Introduction

    Polyhydroxyakanoate (PHA):

    Structure, Biosynthesis

    Production

  • Aimofthissubject

  • Results

  • Summary


Polyhydroxyakanoate pha
Polyhydroxyakanoate biopolymers: The PHB (PHA)

  • PHAs are linear polyesters

  • Manyprokaryotescansynthesizeandstore PHAs in form ofgranules (upto 80 % of total bacterialweight)

  • PHAs havesimilarmechanicalpropertiestoconventionalplastics

  • Theyaregenerallybiodegradable


Polyhydroxyakanoate pha1
Polyhydroxyakanoate biopolymers: The PHB (PHA)

  • The biosynthesis of PHA :

    certain deficiency conditions and

    excess supply of carbon sources

  • The mostcommon/produced form of PHA ispolyhydroxybutyrate, PHB

  • Likepolypropyleneorpolyethylene.

PHB


Biosynthesis of phb
Biosynthesis biopolymers: The PHB of PHB


Phb production
PHB biopolymers: The PHB Production

  • Productioncostsareheigh:

     PHB accumulateintercellularly:

    - Useof a solvent

    - thermal treatment of biomass, enzymatic digestion and washing with an

    surfactant

    a self-disruptivestrain.


  • The biopolymers: The PHB lysissystemofBacillusamyloliquefaciens

  • phage was insertedintoshuttlevector

  • The expression of a target gene is inhibited by

  • glucose

  •  When the glucose concentration approached

  • zero, self-disruption is induced


Phb production1
PHB biopolymers: The PHB Production

  • Productioncostsareheigh:

    carbonsources

    The carbon source could account for 25–45% of the total production costs

Cheapercarbonsources


Phb production2
PHB biopolymers: The PHB Production

  • Agroindustrial wastesareattractivecandidatesassubstrates:

     lowpricesandheighavailability

     solving an environmental problem

  • A variety of microorganisms are able to produce PHB from diverse agroindustrial wastes:

    Methylobacteriumrhodesianum(Borman et al., 1999)

    Cupriavidusnecator(Cavalheiro et al., 2009)

     E. coli CT1061 (Nikelet al., 2008)


Glycerol is carbon source
Glycerol biopolymers: The PHB iscarbonsource

  • Crudeglycerolis a co-product in theproductionofbiodiesel

  • Glycerolis an importantindustrialfeedstock :

    - food, drugs, cosmetics, pharmaceuticals, textile andtobaccoindustries

  • Glycerolcanbeusedascarbonsource in microbiologicalprocesses

  • Polyhydroxyakanoate (PHA) productionis an interestingbiologicaltransformationofglycerol.


Phb production3
PHB biopolymers: The PHB Production

  • Currently, PHB is produced at an industrial scale using Gram negative bacteria

     lipopolysaccharides (LPS) which co-purify with the PHAs and induce a strong immunogenic reaction (Valappil et al., 2007)

Bacillusmegaterium


Aim biopolymers: The PHB

  • Techno-economicanalysisof PHB production:

    - Glyceroltransformationinto PHB (usingBacillusmegaterium)

    - Glycerolor Glucose assubstrate

    - Productivityat different conditions


Results
Results biopolymers: The PHB

  • Different initialconcentrationofglycerol

PHB Production

Biomass

The fermentation conditions:

temperature 30 °C, air flow 12 l/min and uncontrolled pH


Results1
Results biopolymers: The PHB

  • Different temperaturesoffermentation

PHB Production

Biomass

The fermentation conditions:

initial glycerol concentration of 20 g/l, air flow 12 l/min, and uncontrolled pH


Results2
Results biopolymers: The PHB

  • PHB productionusingglucoseorglycerol

VerySimilaryields


Results3
Results biopolymers: The PHB

  • Batch cultivation:

    The fermentations to produce PHB were carried out for 42 h in a 3.7 l Lab Fermen


Results4
Results biopolymers: The PHB

  • Economicanalysis:

  • The current sale prices are between 3.1 and 4.4 USD/kg


Summary
Summary biopolymers: The PHB

  • PHAs are linear polyestersandcanbesynthesizedanditnracellularlystoredbymanyprokaryotes.

  • The mostproduced form of PHA is PHB

  • PHAs havesimilarmechanicalpropertiestoconventionalplastics

  • Productioncostsareheigh

  • PHB ProductionusingB. megaterium, andglycerolascarbonsource

  • The results confirm the ability of B. megaterium to use glycerol as the only carbon source.


References
References biopolymers: The PHB

  • Valorization of glycerol through the production of biopolymers: The PHB caseusingBacillusmegaterium, Javier M. Naranjo, John A. Posada, Juan C. Higuita, Carlos A. Cardona, Bioresource Technology 133 (2013) 38–44.

  • Polyhydroxybutyratesynthesis on biodieselwastewaterusingmixedmicrobialconsortia, Zachary T. Dobroth a, Shengjun Hub, Erik R. Coats a, Armando G. McDonaldBioresource Technology 102 (2011) 3352–3359

  • Large-scale production and efficient recovery of PHB with desirable material properties, from the newly characterised Bacillus cereus SPV, Valappil SP, Misra SK, Boccaccini AR, Keshavarz T, Bucke C, Roy I., J Biotechnol. 2007 Nov 1;132(3):251-8

  • Construction of self-disruptive Bacillus megaterium in response to substrate exhaustion for polyhydroxybutyrate production. Hori K, Kaneko M, Tanji Y, Xing XH, Unno H. Graduate School of Bioscience and Biotechnology, 4259 Nagatsuta-cho, Midori-ku, Yokohama 226-8501, Japan.


THANK YOU biopolymers: The PHB


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