Tumor immunotherapy. 张沛. 张书铭. 张黎明. 赵宸. 赵世刚. Tumour Immunotherapy: questions. Can immune stimulators combat cancer? Which forms of immunotherapy can be used? Is vaccination effective against established tumours? Can anti-tumour responses be generated in vitro ?
Conjugated to toxin / isotope
NKN.o 1 ‘Passive’ immunotherapy
N.o 2Heat Shock Proteins for Therapy
HSPs protect the delicate functions of the cell.
Hsp70 or gp96 / peptide complex
tumour peptides presented to CTL / NK cells via HLA Class I
Transporter Associated with Peptide processing
How is the anti-tumour effect produced?
Peripheral blood from CML patient
Isolate HSP complexes from tumour cells
Load APCwith HSP complexes in vitro
Co-culture with patient T cells and expand effectors for infusion into patient
Immunize patient directly with tumour antigen-primed APC
Immunized with PBS ()
40 µg HSP70 from liver ()
20 µg HSP70 A20 cells ()
40 µg HSP70 A20 cells ()
Pulsed with target antigens
Stimulated to maturation and inoculated back into patient
Tumour-specific immune responses measuredDC-based therapy
Currently in Phase II and Phase III trials for melanoma, prostatic carcinoma and lymphoma.
Ex vivo GM-CSF + IL-4
Ex vivo GM-CSF +
Flt-3 + TNF
Dendritic cell sources for therapy
Copland et al (2005) Cancer Immunol. Immunother. 54:297
Induce, activate or multiply the anti-tumor effective cells invitro.Then transfuse the cells to thepatients to cause anti-tumor function .
The mechanism of cytokine therapy for tumor is that after being injected into body , the cytokine can regulate and increase the activity of immune cells ,and have enhance anti-tumor effect.
So far, IL-2,IFN-r,TNF-a and CSF have been used for tumor treatment .
Tumor cells may lose some genes or express abnormal genes . Transduce target gene to body and restore the function of tumor cells help them to express normal products.
New pathways--gene therapy vaccine
Tumor ‡ Death vaccine
Thank you! vaccine